| dc.creator |
Martínez-Abarca, Francisco |
|
| dc.creator |
Toro, Nicolás |
|
| dc.date |
2008-04-23T10:19:48Z |
|
| dc.date |
2008-04-23T10:19:48Z |
|
| dc.date |
2000 |
|
| dc.date.accessioned |
2017-01-31T01:03:09Z |
|
| dc.date.available |
2017-01-31T01:03:09Z |
|
| dc.identifier |
Nucleic Acids Research, 2000 November 1; 28(21): 4397–4402 |
|
| dc.identifier |
0305-1048 |
|
| dc.identifier |
http://hdl.handle.net/10261/3691 |
|
| dc.identifier |
10.1093/nar/28.21.4397 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3691 |
|
| dc.description |
Final full-text version of the paper available at: http://nar.oxfordjournals.org/cgi/content/abstract/28/21/4397 .-- Copyright © by Oxford University Press. -- Http://nar.oxfordjournals.org/ |
|
| dc.description |
RmInt1 is a group II intron of Sinorhizobium meliloti which was initially found within the insertion sequence ISRm2011-2. Although the RmInt1 intron-encoded protein lacks a recognizable endonuclease domain, it is able to mediate insertion of RmInt1 at an intron-specific location in intronless ISRm2011-2 recipient DNA, a phenomenon termed homing. Here we have characterized three additional insertion sites of RmInt1 in the genome of S.meliloti. Two of these sites are within IS elements closely related to ISRm2011-2, which appear to form a characteristic group within the IS630-Tc1 family. The third site is in the oxi1 gene, which encodes a putative oxide reductase. The newly identified integration sites contain conserved intron-binding site (IBS1 and IBS2) and δ′ sequences (14 bp). The RNA of the intron-containing oxi1 gene is able to splice and the oxi1 site is a DNA target for RmInt1 transposition in vivo. Ectopic transposition of RmInt1 into the oxi1 gene occurs at 20-fold lower efficiency than into the homing site (ISRm2011-2) and is independent of the major RecA recombination pathway. The possibility that transposition of RmInt1 to the oxi1 site occurs by reverse splicing into DNA is discussed. |
|
| dc.description |
This work was supported by grant BIO99-0905 from the Comisión Asesora de Investigación Científica y Técnica and by grant BIO4-CT98-0483 from the Biotechnology Programme of the EU. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
22195 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Oxford University Press |
|
| dc.rights |
openAccess |
|
| dc.title |
RecA-independent ectopic transposition in vivo of a bacterial group II intron |
|
| dc.type |
Artículo |
|