| dc.creator |
Martínez-Abarca, Francisco |
|
| dc.creator |
Barrientos-Durán, Antonio |
|
| dc.creator |
Fernández-López, Manuel |
|
| dc.creator |
Toro, Nicolás |
|
| dc.date |
2008-04-23T07:31:35Z |
|
| dc.date |
2008-04-23T07:31:35Z |
|
| dc.date |
2004-05-20 |
|
| dc.date.accessioned |
2017-01-31T01:03:07Z |
|
| dc.date.available |
2017-01-31T01:03:07Z |
|
| dc.identifier |
Nucleic Acids Research, 2004; 32(9): 2880–2888 |
|
| dc.identifier |
0305-1048 |
|
| dc.identifier |
http://hdl.handle.net/10261/3684 |
|
| dc.identifier |
10.1093/nar/gkh616 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3684 |
|
| dc.description |
Final full-text version of the paper available at: http://nar.oxfordjournals.org/cgi/content/full/32/9/2880 .-- Copyright © by Oxford University Press. -- Http://nar.oxfordjournals.org/ |
|
| dc.description |
Sinorhizobium meliloti RmInt1 is an ef®cient mobile
group II intron that uses an unknown reverse transcriptase
priming mechanism as the intron ribonucleoprotein
complex can reverse splice into DNA
target substrates but cannot carry out site-speci®c
second strand cleavage due to the lack of a Cterminal
DNA endonuclease domain. We show here
that, like other mobile group II introns, RmInt1
moves around by an ef®cient RNA-based retrohoming
mechanism. We found evidence of two distinct
RmInt1 retrohoming pathways for mobility
depending on the orientation of the target site relative
to the direction of DNA replication. The preferred
retrohoming pathway is consistent with
reverse splicing of the intron RNA into singlestranded
DNA at a replication fork, using a nascent
lagging DNA strand as the primer for reverse transcription.
This strand bias is the opposite of that
reported for mobility of the lactococcal Ll.ltrB intron
in the absence of second strand cleavage. The
mobility mechanism found here for RmInt1 may
be used for dissemination by many bacterial group
II introns encoding proteins lacking the DNA
endonuclease domain. |
|
| dc.description |
This work was funded by
research project BIO2002-02579 from the Ministerio de
Ciencia y Tecnología. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
264052 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Oxford University Press |
|
| dc.rights |
openAccess |
|
| dc.title |
The RmInt1 group II intron has two different retrohoming pathways for mobility using predominantly the nascent lagging strand at DNA replication forks for priming |
|
| dc.type |
Artículo |
|