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Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre

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dc.creator Yruela Guerrero, Inmaculada
dc.creator García-Rubio, Inés
dc.creator Roncel Gil, Mercedes
dc.creator Martínez, Jesús I.
dc.creator Ramiro Pascual, María Victoria
dc.creator Ortega, José M.
dc.creator Alonso, Pablo J.
dc.creator Picorel Castaño, Rafael
dc.date 2008-04-16T15:43:20Z
dc.date 2008-04-16T15:43:20Z
dc.date 2003
dc.date.accessioned 2017-01-31T01:02:28Z
dc.date.available 2017-01-31T01:02:28Z
dc.identifier Photochem. Photobiol. Sci., 2003, (4),437-442
dc.identifier http://hdl.handle.net/10261/3630
dc.identifier 10.1039/b300187c
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3630
dc.description The definitive version is available at: http://www.rsc.org/publishing/journals/PP/
dc.description The detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1–D2–Cyt b559 complex preparations. Various n-dodecyl--D-maltoside concentrations from 0 to 0.2%(w/v) were used to stabilise the D1–D2–Cyt b559 complexes. Low spin heme EPR spectra were obtained but the gz feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (Em=+123–150 mV) in all the D1–D2–Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein.
dc.description I. G.-R. was recipient of a fellowship from the Ministry of Education and Culture of Spain. This work was supported by the Dirección General de Investigación Científica y Técnica (Grants PB98-1632 to R.P. and PB97-1135 to J.M.O.) and by the Diputación General de Aragón (Projects P17/98 to P.J.A. and P111/2001 to J.I.M.).
dc.description Peer reviewed
dc.format 158025 bytes
dc.format application/pdf
dc.language eng
dc.publisher Royal Society of Chemistry (Great Britain)
dc.rights openAccess
dc.subject cytochrome b559
dc.subject detergent
dc.subject electron paramagnetic resonance
dc.subject photosystem II
dc.subject reaction centre
dc.subject redox potential
dc.title Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre
dc.type Artículo


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