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Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system

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dc.creator Sanz, Libia
dc.creator Bazaa, Amine
dc.creator Marrakchi, Naziha
dc.creator Pérez, Alicia
dc.creator Chenik, Mehdi
dc.creator Bel Lasfer, Zakaria
dc.creator El Ayeb, Mohamed
dc.creator Calvete, Juan J.
dc.date 2008-04-15T11:42:48Z
dc.date 2008-04-15T11:42:48Z
dc.date 2006-03-28
dc.date.accessioned 2017-01-31T01:02:19Z
dc.date.available 2017-01-31T01:02:19Z
dc.identifier Biochem J. 2006 April 15; 395(Pt 2): 385–392
dc.identifier 1470-8728
dc.identifier http://hdl.handle.net/10261/3589
dc.identifier 10.1042/BJ20051678
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3589
dc.description Copyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htm
dc.description We report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs of short disintegrins and those coding for precursors of dimeric disintegrin chains. The Macrovipera lebetina transmediterranea dimeric disintegrin reported to inhibit the laminin-binding integrins α3β1, α6β1 and α7β1 was analysed using a proteomic approach and was shown to bear MLD (Met-Leu-Asp) and VGD (Val-Gly-Asp) motifs. The results highlight the fact that disintegrins have evolved a restricted panel of integrin-blocking sequences that segregate with defined branches of the phylogenetic tree of the integrin α-chains, providing novel insights into the evolutionary adaptation of the snake venom antagonists to the ligand-binding sites of their target integrin receptors.
dc.description This study has been partly financed by grant BFU2004-01432/BMC from the Ministerio de Educación y Ciencia, Madrid, Spain, and by the Spanish–Tunisian Cooperation Programme 29p/02 from the Agencia Española de Cooperación Internacional (AECI).
dc.description Peer reviewed
dc.format 763802 bytes
dc.format application/pdf
dc.language eng
dc.publisher Biochemical Society
dc.rights openAccess
dc.subject Cerastes vipera
dc.subject Macrovipera lebetina transmediterranea
dc.subject Integrin inhibition
dc.subject Snake venom disintegrin
dc.subject Snake venomics
dc.subject Toxin evolution
dc.title Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
dc.type Artículo


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