| dc.contributor |
Ministero delle Politiche Agricole Alimentari e Forestali (Italia) |
|
| dc.creator |
Lorito, Matteo |
|
| dc.creator |
Scala, Felice |
|
| dc.creator |
García Fernández, Irene |
|
| dc.creator |
Pintor-Toro, José Antonio |
|
| dc.date |
2008-04-14T12:16:03Z |
|
| dc.date |
2008-04-14T12:16:03Z |
|
| dc.date |
1998-07-07 |
|
| dc.date.accessioned |
2017-01-31T01:02:17Z |
|
| dc.date.available |
2017-01-31T01:02:17Z |
|
| dc.identifier |
Proceedings of the National Academy of Sciences 95(14): 7860–7865 (1998) |
|
| dc.identifier |
1091-6490 |
|
| dc.identifier |
http://hdl.handle.net/10261/3569 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3569 |
|
| dc.description |
Lorito, Matteo et al.-- |
|
| dc.description |
Disease resistance in transgenic plants has been improved, for the first time, by the insertion of a gene from a biocontrol fungus. The gene encoding a strongly antifungal endochitinase from the mycoparasitic fungus Trichoderma harzianum was transferred to tobacco and potato. High expression levels of the fungal gene were obtained in different plant tissues, which had no visible effect on plant growth and development. Substantial differences in endochitinase activity were detected among transformants. Selected transgenic lines were highly tolerant or completely resistant to the foliar pathogens Alternaria alternata, A. solani, Botrytis cinerea, and the soilborne pathogen Rhizoctonia solani. The high level and the broad spectrum of resistance obtained with a single chitinase gene from Trichoderma overcome the limited efficacy of transgenic expression in plants of chitinase genes from plants and bacteria. These results demonstrate a rich source of genes from biocontrol fungi that can be used to control diseases in plants. |
|
| dc.description |
S.L.W. has been supported by grants from Consorzio per la Ricerca Applicata in Agricoltura (CRAA) and Piano Nazionale Biotecnologie Vegetali del Mi.R.A.A.F., and M.L. has been supported in part by a Fullbright fellowship for research. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
368446 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
National Academy of Sciences (U.S.) |
|
| dc.rights |
openAccess |
|
| dc.title |
Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens |
|
| dc.type |
Artículo |
|