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Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase

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dc.contributor Ministerio de Educación y Cultura (España)
dc.contributor Junta de Andalucía
dc.contributor European Commission
dc.contributor Rhône-Poulenc
dc.creator Romero, Francisco
dc.creator Multon, Marie-Christine
dc.creator Ramos-Morales, Francisco
dc.creator Domínguez, África
dc.creator Bernal, Juan A.
dc.creator Pintor-Toro, José Antonio
dc.creator Tortolero, María
dc.date 2008-04-14T10:54:58Z
dc.date 2008-04-14T10:54:58Z
dc.date 2001
dc.date.accessioned 2017-01-31T01:02:16Z
dc.date.available 2017-01-31T01:02:16Z
dc.identifier Nucleic Acids Research 29(6): 1300–1307 (2001)
dc.identifier 1362-4962
dc.identifier http://hdl.handle.net/10261/3563
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3563
dc.description 8 pages, 5 figures, 58 references.
dc.description We have previously isolated the hpttg proto-oncogene, which is expressed in normal tissues containing proliferating cells and in several kinds of tumors. In fact, expression of hPTTG correlates with cell proliferation in a cell cycle-dependent manner. Recently it was reported that PTTG is a vertebrate analog of the yeast securins Pds1 and Cut2, which are involved in sister chromatid separation. Here we show that hPTTG binds to Ku, the regulatory subunit of the DNA-dependent protein kinase (DNA-PK). hPTTG and Ku associate both in vitro and in vivo and the DNA-PK catalytic subunit phosphorylates hPTTG in vitro. Furthermore, DNA double-strand breaks prevent hPTTG–Ku association and disrupt the hPTTG–Ku complexes, indicating that genome damaging events, which result in the induction of pathways that activate DNA repair mechanisms and halt cell cycle progression, might inhibit hPTTG–Ku interaction in vivo. We propose that hPTTG might connect DNA damage-response pathways with sister chromatid separation, delaying the onset of mitosis while DNA repair occurs.
dc.description This work was supported by grants from Ministerio de Educación y Cultura (MEC) of Spain (SAF99-0125-C03-01), from DGUI of the Junta de Andalucía and from Rhône-Poulenc Rorer (Paris, France). F.R. was supported by postdoctoral fellowships from the EU and Spanish MEC.
dc.description Peer reviewed
dc.format 270304 bytes
dc.format application/pdf
dc.language eng
dc.publisher Oxford University Press
dc.rights openAccess
dc.title Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase
dc.type Artículo


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