| dc.creator |
Maldonado-Barragán, Antonio |
|
| dc.creator |
Ruiz-Barba, José Luis |
|
| dc.creator |
Jiménez Díaz, Rufino |
|
| dc.date |
2008-04-08T07:28:47Z |
|
| dc.date |
2008-04-08T07:28:47Z |
|
| dc.date |
2003-01 |
|
| dc.date.accessioned |
2017-01-31T01:01:36Z |
|
| dc.date.available |
2017-01-31T01:01:36Z |
|
| dc.identifier |
Applied and Environmental Microbiology 69(1): 383–389 (2003) |
|
| dc.identifier |
1098-5336 |
|
| dc.identifier |
http://hdl.handle.net/10261/3462 |
|
| dc.identifier |
10.1128/AEM.69.1.383-389.2003 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3462 |
|
| dc.description |
A new, coculture-inducible two-peptide bacteriocin named plantaricin NC8 (PLNC8) was isolated from
Lactobacillus plantarum NC8 cultures which had been induced with Lactococcus lactis MG1363 or Pediococcus
pentosaceus FBB63. This bacteriocin consists of two distinct peptides, named and , which were separated by
C2-C18 reverse-phase chromatography and whose complementary action is necessary for full plantaricin NC8
activity. N-terminal sequencing of both purified peptides showed 28 and 34 amino acids residues for PLNC8
and PLNC8 , respectively, which showed no sequence similarity to other known bacteriocins. Mass spectrometry
analysis showed molecular masses of 3,587 Da ( ) and 4,000 Da ( ). The corresponding genes, designated
plNC8A and plNC8B, were sequenced, and their nucleotide sequences revealed that both peptides are produced
as bacteriocin precursors of 47 and 55 amino acids, respectively, which include N-terminal leader sequences
of the double-glycine type. The mature and peptides contain 29 and 34 amino acids, respectively. An open
reading frame, orfC, which encodes a putative immunity protein was found downstream of plNC8B and
overlapping plNC8A. Upstream of the putative 35 region of plNC8B, two direct repeats of 9 bp were identified,
which agrees with the consensus sequence and structure of promoters of class II bacteriocin operons whose
expression is dependent on an autoinduction mechanism. |
|
| dc.description |
This work was supported by the Spanish Government through
MCYT project AGL2000-1611-CO3-01. A.M. was the recipient of a
grant from MCYT, Spain. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
297743 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
American Society for Microbiology |
|
| dc.relation |
http://dx.doi.org/10.1128/AEM.69.1.383-389.2003 |
|
| dc.rights |
openAccess |
|
| dc.title |
Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin from Lactobacillus plantarum NC8 |
|
| dc.type |
Artículo |
|