| dc.creator |
Ingrosso, Loredana |
|
| dc.creator |
Novoa, Beatriz |
|
| dc.creator |
Aranguren, Raquel |
|
| dc.creator |
Figueras, Antonio |
|
| dc.date |
2008-04-07T07:12:41Z |
|
| dc.date |
2008-04-07T07:12:41Z |
|
| dc.date |
2006-06-15 |
|
| dc.date.accessioned |
2017-01-31T01:01:35Z |
|
| dc.date.available |
2017-01-31T01:01:35Z |
|
| dc.identifier |
BMC Veterinary Research 2: 21 (2006) |
|
| dc.identifier |
1746-6148 |
|
| dc.identifier |
http://hdl.handle.net/10261/3450 |
|
| dc.identifier |
10.1186/1746-6148-2-21 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3450 |
|
| dc.description |
7 páginas, 1 tabla. 3 figura.--Loredana Ingrosso ... et al.-- This article is available from: http://www.biomedcentral.com/1746-6148/2/21 |
|
| dc.description |
[Background] Scrapie and bovine spongiform encephalopathy (BSE) belongs to the group of animal transmissible
spongiform encephalopathy (TSE). BSE epidemic in the UK and elsewhere in Europe has been linked to the use
of bovine meat and bone meals (MBM) in the feeding of cattle. There is concern that pigs, poultry and fish bred
for human consumption and fed with infected MBM would eventually develop BSE or carry residual infectivity
without disease. Although there has been no evidence of infection in these species, experimental data on the
susceptibility to the BSE agent of farm animals other than sheep and cow are limited only to pigs and domestic
chicken. In the framework of a EU-granted project we have challenged two species of fish largely used in human
food consumption, rainbow trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus), with a mouseadapted
TSE strain (scrapie 139A), to assess the risk related to oral consumption of TSE contaminated food. In
trout, we also checked the "in vitro" ability of the pathological isoform of the mouse prion protein (PrPSc) to cross
the intestinal epithelium when added to the mucosal side of everted intestine. |
|
| dc.description |
[Results] Fish challenged with a large amount of scrapie mouse brain homogenate by either oral or parenteral
routes, showed the ability to clear the majority of infectivity load. None of the fish tissues taken at different time
points after oral or parenteral inoculation was able to provoke scrapie disease after intracerebral inoculation in
recipient mice. However, a few recipient mice were positive for PrPSc and spongiform lesions in the brain. We
also showed a specific binding of PrPSc to the mucosal side of fish intestine in the absence of an active uptake of
the prion protein through the intestinal wall. |
|
| dc.description |
[Conclusion] These results indicate that scrapie 139A, and possibly BSE, is quickly removed from fish tissues
despite evidence of a prion like protein in fish and of a specific binding of PrPSc to the mucosal side of fish intestine. |
|
| dc.description |
This study was funded through the EU grant FAIR CT97 3308 and the EU
grant QLK5-2002-00866. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
1239023 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
BioMed Central |
|
| dc.relation |
Publisher’s version |
|
| dc.relation |
http://dx.doi.org/10.1186/1746-6148-2-21 |
|
| dc.rights |
openAccess |
|
| dc.title |
Scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish |
|
| dc.type |
Artículo |
|