المستودع الأكاديمي جامعة المدينة

EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

أعرض تسجيلة المادة بشكل مبسط

dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Institut National de la Recherche Agronomique (France)
dc.contributor European Commission
dc.creator Nieto, Cristina
dc.creator Piron, Florence
dc.creator Dalmais, Marion
dc.creator Gómez-Guillamón, M. L.
dc.creator Truniger, Verónica
dc.creator Gómez, Pedro
dc.creator García-Mas, Jordi
dc.date 2008-04-02T07:57:12Z
dc.date 2008-04-02T07:57:12Z
dc.date 2007-06-21
dc.date.accessioned 2017-01-31T01:01:25Z
dc.date.available 2017-01-31T01:01:25Z
dc.identifier BMC Plant Biol. 2007; 7: 34
dc.identifier 1471-2229
dc.identifier http://hdl.handle.net/10261/3409
dc.identifier 10.1186/1471-2229-7-34
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3409
dc.description This article is available from: http://www.biomedcentral.com/1471-2229/7/34
dc.description [Background] Translation initiation factors of the 4E and 4G protein families mediate resistance to several RNA plant viruses in the natural diversity of crops. Particularly, a single point mutation in melon eukaryotic translation initiation factor 4E (eIF4E) controls resistance to Melon necrotic spot virus (MNSV) in melon. Identification of allelic variants within natural populations by EcoTILLING has become a rapid genotype discovery method.
dc.description [Results] A collection of Cucumis spp. was characterised for susceptibility to MNSV and Cucumber vein yellowing virus (CVYV) and used for the implementation of EcoTILLING to identify new allelic variants of eIF4E. A high conservation of eIF4E exonic regions was found, with six polymorphic sites identified out of EcoTILLING 113 accessions. Sequencing of regions surrounding polymorphisms revealed that all of them corresponded to silent nucleotide changes and just one to a non-silent change correlating with MNSV resistance. Except for the MNSV case, no correlation was found between variation of eIF4E and virus resistance, suggesting the implication of different and/or additional genes in previously identified resistance phenotypes. We have also characterized a new allele of eIF4E from Cucumis zeyheri, a wild relative of melon. Functional analyses suggested that this new eIF4E allele might be responsible for resistance to MNSV.
dc.description [Conclusion] This study shows the applicability of EcoTILLING in Cucumis spp., but given the conservation of eIF4E, new candidate genes should probably be considered to identify new sources of resistance to plant viruses. Part of the methodology described here could alternatively be used in TILLING experiments that serve to generate new eIF4E alleles.
dc.description This work was supported by grants from Ministerio de Educación y Ciencia (ref. AGL2003-02739) (Spain) and GENOPLANTE (France). Cristina Nieto was in receipt of a Marie Curie Fellowship.
dc.description Peer reviewed
dc.format 745878 bytes
dc.format 164696 bytes
dc.format application/pdf
dc.format application/pdf
dc.language eng
dc.publisher BioMed Central
dc.relation Publisher’s version
dc.rights openAccess
dc.title EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility
dc.type Artículo


الملفات في هذه المادة

الملفات الحجم الصيغة عرض

لا توجد أي ملفات مرتبطة بهذه المادة.

هذه المادة تبدو في المجموعات التالية:

أعرض تسجيلة المادة بشكل مبسط