| dc.creator |
Ciudad, Laura |
|
| dc.creator |
Bellés, Xavier |
|
| dc.creator |
Piulachs, Maria-Dolors |
|
| dc.date |
2008-04-02T07:38:40Z |
|
| dc.date |
2008-04-02T07:38:40Z |
|
| dc.date |
2007-06-22 |
|
| dc.date.accessioned |
2017-01-31T01:01:24Z |
|
| dc.date.available |
2017-01-31T01:01:24Z |
|
| dc.identifier |
BMC Mol Biol. 2007; 8: 53 |
|
| dc.identifier |
1471-2199 |
|
| dc.identifier |
http://hdl.handle.net/10261/3408 |
|
| dc.identifier |
10.1186/1471-2199-8-53 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3408 |
|
| dc.description |
This article is available from: http://www.biomedcentral.com/1471-2199/8/53 |
|
| dc.description |
[Background] Lipophorin receptors (LpRs) have been described in a number of insects, but
functional studies have been reported only in locusts and mosquitoes. The aim of the present work
was to characterize the LpR of the cockroach Blattella germanica, not only molecularly but also
functionally using RNAi techniques, and to place LpRs in a phylogenetical context among
lipoprotein receptors. |
|
| dc.description |
[Results] We cloned a putative LpR from B. germanica (BgLpR) using RT-PCR methods. Two
isoforms of BgLpR that differ from each other by an insertion/deletion of 24 amino acids were
obtained from the fat body and the ovary. A phylogenetical analysis of lipoprotein receptors
showed that BgLpR grouped with other sequences annotated as LpR in a cluster placed as a sister
group of vertebrate low density lipoprotein receptors (LDLR) + lipoprotein receptor-related
proteins 8 (LPR8) + vitellogenin receptors (VgR) + very low density lipoprotein receptors
(VLDLR). The two BgLpR isoforms are expressed in different adult female tissues (fat body, ovary,
brain, midgut, muscle) and in embryos. In ovaries and fat body, the two isoforms are similarly
expressed during the first gonadotrophic cycle. mRNA levels in the fat body increase in parallel to
vitellogenesis, whereas they decrease in the ovaries. BgLpR protein levels increase in parallel to
vitellogenesis in both organs. Treatment with juvenile hormone increases BgLpR protein. RNAi
experiments show that females with lower BgLpR expression have less lipophorin in the growing
oocytes with respect to controls. |
|
| dc.description |
[Conclusion] The two isoforms of BgLpR are structurally similar to other LpRs. Phylogenetical
analyses show that LpRs and the group formed by vertebrate LDLR + LPR8 + VgR + VLDLR,
diverged from a common ancestor and diversified in parallel. The different expression patterns in
the fat body and the ovary, comparing mRNA and protein, indicate that the corresponding
mechanisms regulating BgLpR expression are different. Experiments with JH III suggest that such a
hormone regulates the expression of BgLpR posttranscriptionally. RNAi experiments indicate that
BgLpR is a functional lipophorin receptor. |
|
| dc.description |
Financial support from the Ministry of Education and Science, Spain
(projects BOS2002-03359, BFU2005-00264, AGL2002-01169, AGL2005-
00773) and the Generalitat de Catalunya (2001 SGR 003245) is gratefully
acknowledged. L.C. is recipient of pre-doctoral research grants (I3P) from
CSIC. We thank Prof. Coby Schal (North Carolina State University) for
generous gifts of anti-lipophorin antibody. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
581002 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
BioMed Central |
|
| dc.relation |
Publisher’s version |
|
| dc.rights |
openAccess |
|
| dc.title |
Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors |
|
| dc.type |
Artículo |
|