أعرض تسجيلة المادة بشكل مبسط

dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Gobierno de la Región de Murcia
dc.contributor CSIC-IRTA-UAB-UB - Centre de Recerca Agrigenómica (CRAG)
dc.creator González-Ibeas, Daniel
dc.creator Blanca, José M.
dc.creator Roig, Cristina
dc.creator González-To, Mireia
dc.creator Picó-Silvent, Belén
dc.creator Truniger, Verónica
dc.creator Gómez, Pedro
dc.creator Deleu, Wim
dc.creator Caño-Delgado, Ana I.
dc.creator Arús, Pere
dc.creator Nuez, Fernando
dc.creator García-Mas, Jordi
dc.creator Puigdomènech, Pere
dc.creator Aranda, Miguel A.
dc.date 2008-04-01T07:18:43Z
dc.date 2008-04-01T07:18:43Z
dc.date 2007-09-03
dc.date.accessioned 2017-01-31T01:01:23Z
dc.date.available 2017-01-31T01:01:23Z
dc.identifier BMC Genomics. 2007; 8: 306
dc.identifier 1471-2164
dc.identifier http://hdl.handle.net/10261/3395
dc.identifier 10.1186/1471-2164-8-306
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3395
dc.description This article is available from: http://www.biomedcentral.com/1471-2164/8/306
dc.description [Background] Melon (Cucumis melo L.) is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions.
dc.description [Results] We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs) and 10,614 unclustered sequences (singletons). Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs) and 356 single nucleotide polymorphisms (SNPs) were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs) in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes.
dc.description [Conclusion] The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN) which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of sequences constitutes also the basis for an oligo-based microarray for melon that is being used in experiments to further analyse the melon transcriptome.
dc.description This work was supported by grants from Ministerio de Educación y Ciencia (Spain) (GEN2003-20237-C06) and Consejería de Educación y Cultura (Región de Murcia, Spain) (BIO2005/04-6436). Wim Deleu, Cristina Roig and Daniel Gonzalez-Ibeas are recipient of a postdoctoral fellowship from the Centre de Recerca en Agrigenòmica CSIC-IRTA (Spain), a Juan de la Cierva grant from Ministerio de Educación y Ciencia (Spain) and a predoctoral fellowship from Ministerio de Educación y Ciencia (Spain), respectively.
dc.description Peer reviewed
dc.format 489917 bytes
dc.format 114132 bytes
dc.format application/pdf
dc.format application/pdf
dc.language eng
dc.publisher BioMed Central
dc.relation Publisher’s version
dc.rights openAccess
dc.title MELOGEN: an EST database for melon functional genomics
dc.type Artículo


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