| dc.creator |
Lacalle, Rosa Ana |
|
| dc.creator |
Mira, Emilia |
|
| dc.creator |
Gómez-Moutón, Concepción |
|
| dc.creator |
Jiménez Baranda, Sonia |
|
| dc.creator |
Martínez-Alonso, Carlos |
|
| dc.creator |
Mañes, Santos |
|
| dc.date |
2008-03-31T10:27:04Z |
|
| dc.date |
2008-03-31T10:27:04Z |
|
| dc.date |
2002-04 |
|
| dc.date.accessioned |
2017-01-31T01:01:22Z |
|
| dc.date.available |
2017-01-31T01:01:22Z |
|
| dc.identifier |
The Journal of Cell Biology, volume 157, number 2, april 15, 2002, pp. 277–289 |
|
| dc.identifier |
0021-9525 |
|
| dc.identifier |
http://hdl.handle.net/10261/3387 |
|
| dc.identifier |
10.1083/jcb.200109031 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3387 |
|
| dc.description |
Copyright © by The Rockefeller University Press |
|
| dc.description |
Cell signaling does not occur randomly over the cell surface, but is integrated within cholesterol-enriched membrane domains, termed rafts. By targeting SHP-2
to raft domains or to a non-raft plasma membrane fraction, we studied the functional role of rafts in signaling. Serumdepleted, nonattached cells expressing the raft SHP-2 form, but not non-raft SHP-2, display signaling events resembling
those observed after fibronectin attachment, such as 1 integrin clustering,
397 Y-FAK phosphorylation, and ERK
activation, and also increases Rho-GTP levels. Expression of C the dominant negative N19Rho abrogates raft-SHP-2–induced
signaling, suggesting that Rho activation is a downstream event in SHP-2 signaling. Expression of a catalytic inactive
SHP-2 mutant abrogates the adhesion-induced feedback inhibition of Rho activity, suggesting that SHP-2 contributes
to adhesion-induced suppression of Rho activity. Because raft recruitment of SHP-2 occurs physiologically after cell
attachment, these results provide a mechanism by which SHP-2 may influence cell adhesion and migration by spatially regulating Rho activity. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
696619 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Rockefeller University Press |
|
| dc.rights |
openAccess |
|
| dc.title |
Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation |
|
| dc.type |
Artículo |
|