| dc.creator |
Fredriksson, Åsa |
|
| dc.creator |
Ballesteros, Manuel |
|
| dc.creator |
Dukan, Sam |
|
| dc.creator |
Nyström, Thomas |
|
| dc.date |
2008-03-31T07:29:59Z |
|
| dc.date |
2008-03-31T07:29:59Z |
|
| dc.date |
2005-06 |
|
| dc.date.accessioned |
2017-01-31T01:01:21Z |
|
| dc.date.available |
2017-01-31T01:01:21Z |
|
| dc.identifier |
Journal Bacteriology 187(12): 4207–4213 (2005) |
|
| dc.identifier |
1098-5530 |
|
| dc.identifier |
http://hdl.handle.net/10261/3378 |
|
| dc.identifier |
10.1128/JB.187.12.4207–4213.2005 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3378 |
|
| dc.description |
Protein carbonylation is an irreversible oxidative modification that increases during organism aging and
bacterial growth arrest. We analyzed whether the heat shock regulon has a role in defending Escherichia coli
cells against this deleterious modification upon entry into stationary phase. Providing the cell with ectopically
elevated levels of the heat shock transcription factor, 32, effectively reduced stasis-induced carbonylation.
Separate overproduction of the major chaperone systems, DnaK/DnaJ and GroEL/GroES, established that the
former of these is more important in counteracting protein carbonylation. Deletion of the heat shock proteases
Lon and HslVU enhanced carbonylation whereas a clpP deletion alone had no effect. However, ClpP appears
to have a role in reducing protein carbonyls in cells lacking Lon and HslVU. Proteomic immunodetection of
carbonylated proteins in the wild-type, lon, and hslVU strains demonstrated that the same spectrum of proteins
displayed a higher load of carbonyl groups in the lon and hslVU mutants. These proteins included the -subunit
of RNA polymerase, elongation factors Tu and G, the E1 subunit of the pyruvate dehydrogenase complex,
isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, and serine hydroxymethyltranferase. |
|
| dc.description |
This work was sponsored by grants from the Swedish Natural Research
Council and the Foundation for Strategic Research in Sweden
and an award from the Go¨ran Gustafsson Foundation for Scientific
Research in Molecular Biology. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
242551 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
American Society for Microbiology |
|
| dc.relation |
http://dx.doi.org/10.1128/JB.187.12.4207-4213.2005 |
|
| dc.rights |
closedAccess |
|
| dc.title |
Defense against Protein Carbonylation by DnaK/DnaJ and Proteases of the Heat Shock Regulon |
|
| dc.type |
Artículo |
|