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Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter

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dc.creator Díaz-Martín, J.
dc.creator Prieto-Dapena, P.
dc.creator Jordano, Juan
dc.creator Espinosa-Vázquez, José Manuel
dc.creator Almoguera, Concepción
dc.date 2008-03-28T07:11:08Z
dc.date 2008-03-28T07:11:08Z
dc.date 2005-10-21
dc.date.accessioned 2017-01-31T01:01:15Z
dc.date.available 2017-01-31T01:01:15Z
dc.identifier Plant Physiology 139:1483-1494 (2005)
dc.identifier 0032-0889
dc.identifier http://hdl.handle.net/10261/3347
dc.identifier 10.1104/pp.105.069963.
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3347
dc.description Hahsp17.6G1 is the promoter of a small heat stress protein (sHSP) from sunflower (Helianthus annuus) that is activated during zygotic embryogenesis, but which does not respond to heat stress. We report here the cloning of a transcription factor (TF), sunflower drought-responsive element binding factor 2 (HaDREB2), by one-hybrid interaction with functional cis-elements in Hahsp17.6G1. We have analyzed the functional interaction between HaDREB2 and a second transcription factor, sunflower heat stress factor A9 (HaHSFA9), which was previously assigned to the regulation of Hahsp17.6G1. HaDREB2 and HaHSFA9 synergistically trans-activate the Hahsp17.6G1 promoter in bombarded sunflower embryos. This synergistic interaction is heat stress factor (HSF) specific and requires the binding of both factors to the promoter. The C-terminal region of HaHSFA9 is sufficient for the HSF specificity. Our results represent an example of a functional interaction between members of the Apetala 2 (HaDREB2) and HSF (HaHSFA9) families of transcription factors. We suggest new roles in zygotic embryogenesis for specific members of the AP2 transcription factor family.
dc.description Peer reviewed
dc.format 488602 bytes
dc.format 580674 bytes
dc.format 31158 bytes
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.language eng
dc.publisher American Society of Plant Biologists
dc.relation http://www.plantphysiol.org/cgi/reprint/139/3/1483
dc.rights closedAccess
dc.subject Activator Fuction
dc.subject Shock Proteins
dc.subject Expression
dc.subject Arabidopsis
dc.subject Embryogenesis
dc.subject Domain
dc.subject Identification
dc.subject Tolerance
dc.subject Sunflower
dc.subject Nerwork
dc.title Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter
dc.type Artículo


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