المستودع الأكاديمي جامعة المدينة

Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement

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dc.creator Lacalle, Rosa Ana
dc.creator Peregil, Rosa M.
dc.creator Albar, Juan Pablo
dc.creator Merino Plaza, Ernesto
dc.creator Martínez-Alonso, Carlos
dc.creator Mérida, Isabel
dc.creator Mañes, Santos
dc.date 2008-03-18T12:13:38Z
dc.date 2008-03-18T12:13:38Z
dc.date 2007-12
dc.date.accessioned 2017-01-31T01:00:46Z
dc.date.available 2017-01-31T01:00:46Z
dc.identifier The Journal of Cell Biology, Vol. 179, No. 7, pp. 1539-1553
dc.identifier 0021-9525
dc.identifier http://hdl.handle.net/10261/3242
dc.identifier 10.1083/jcb.200705044
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3242
dc.description Copyright by The Rockefeller University Press
dc.description Directional cell movement in response to external chemical gradients requires establishment of front–rear asymmetry, which distinguishes an up-gradient protrusive leading edge, where Rac-induced F-actin polymerization takes place, and a down-gradient retractile tail (uropod in leukocytes), where RhoA-mediated actomyosin contraction occurs. The signals that govern this spatial and functional asymmetry are not entirely understood. We show that the human type I phosphatidylinositol 4-phosphate 5-kinase isoform β (PIPKIβ) has a role in organizing signaling at the cell rear. We found that PIPKIβ polarized at the uropod of neutrophil-differentiated HL60 cells. PIPKIβ localization was independent of its lipid kinase activity, but required the 83 C-terminal amino acids, which are not homologous to other PIPKI isoforms. The PIPKIβ C terminus interacted with EBP50 (4.1-ezrin-radixin-moesin (ERM)-binding phosphoprotein 50), which enabled further interactions with ERM proteins and the Rho-GDP dissociation inhibitor (RhoGDI). Knockdown of PIPKIβ with siRNA inhibited cell polarization and impaired cell directionality during dHL60 chemotaxis, suggesting a role for PIPKIβ in these processes.
dc.description Peer reviewed
dc.format 404731 bytes
dc.format application/pdf
dc.language eng
dc.publisher Rockefeller University Press
dc.rights openAccess
dc.title Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement
dc.type Artículo


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