| dc.creator |
Lacalle, Rosa Ana |
|
| dc.creator |
Peregil, Rosa M. |
|
| dc.creator |
Albar, Juan Pablo |
|
| dc.creator |
Merino Plaza, Ernesto |
|
| dc.creator |
Martínez-Alonso, Carlos |
|
| dc.creator |
Mérida, Isabel |
|
| dc.creator |
Mañes, Santos |
|
| dc.date |
2008-03-18T12:13:38Z |
|
| dc.date |
2008-03-18T12:13:38Z |
|
| dc.date |
2007-12 |
|
| dc.date.accessioned |
2017-01-31T01:00:46Z |
|
| dc.date.available |
2017-01-31T01:00:46Z |
|
| dc.identifier |
The Journal of Cell Biology, Vol. 179, No. 7, pp. 1539-1553 |
|
| dc.identifier |
0021-9525 |
|
| dc.identifier |
http://hdl.handle.net/10261/3242 |
|
| dc.identifier |
10.1083/jcb.200705044 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3242 |
|
| dc.description |
Copyright by The Rockefeller University Press |
|
| dc.description |
Directional cell movement in response to external chemical gradients requires establishment of front–rear
asymmetry, which distinguishes an up-gradient protrusive leading edge, where Rac-induced F-actin polymerization
takes place, and a down-gradient retractile tail (uropod in leukocytes), where RhoA-mediated actomyosin contraction occurs. The signals that govern this spatial
and functional asymmetry are not entirely understood. We show that the human type I phosphatidylinositol 4-phosphate 5-kinase isoform β (PIPKIβ) has a role in
organizing signaling at the cell rear. We found that PIPKIβ polarized at the uropod of
neutrophil-differentiated HL60 cells. PIPKIβ localization was independent of its lipid kinase activity, but required the 83 C-terminal amino acids, which are not homologous to other PIPKI isoforms. The PIPKIβ C terminus interacted with EBP50 (4.1-ezrin-radixin-moesin
(ERM)-binding phosphoprotein 50), which enabled further interactions with ERM proteins and the Rho-GDP dissociation inhibitor (RhoGDI). Knockdown of PIPKIβ with siRNA inhibited cell polarization and impaired cell directionality during dHL60 chemotaxis, suggesting a role for PIPKIβ in these processes. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
404731 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Rockefeller University Press |
|
| dc.rights |
openAccess |
|
| dc.title |
Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement |
|
| dc.type |
Artículo |
|