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Population Genetics of Vibrio vulnificus: Identification of Two Divisions and a Distinct Eel-Pathogenic Clone

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dc.creator Gutacker, Michaela
dc.creator Conza, Nadine
dc.creator Benagli, Cinzia
dc.creator Pedroli, Ambra
dc.creator Bernasconi, Marco Valerio
dc.creator Permin, Lise
dc.creator Aznar, Rosa
dc.creator Piffaretti, Jean-Claude
dc.date 2008-02-25T10:40:17Z
dc.date 2008-02-25T10:40:17Z
dc.date 2003-06
dc.date.accessioned 2017-01-31T01:00:22Z
dc.date.available 2017-01-31T01:00:22Z
dc.identifier Applied and Environmental Microbiology 69 (6) : 3203-3212 (2003)
dc.identifier http://hdl.handle.net/10261/3058
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3058
dc.description Genetic relationships among 62 Vibrio vulnificus strains of different geographical and host origins were analyzed by multilocus enzyme electrophoresis (MLEE), random amplification of polymorphic DNA (RAPD), and sequence analyses of the recA and glnA genes. Out of 15 genetic loci analyzed by MLEE, 11 were polymorphic. Cluster analysis identified 43 distinct electrophoretic types (ETs) separating the V. vulnificus population into two divisions (divisions I and II). One ET (ET 35) included all indole-negative isolates from diseased eels worldwide (biotype 2). A second ET (ET 2) marked all of the strains from Israel isolated from patients who handled St. Peter's fish (biotype 3). RAPD analysis of the 62 V. vulnificus isolates identified 26 different profiles separated into two divisions as well. In general, this subdivision was comparable (but not identical) to that observed by MLEE. Phylogenetic analysis of 543 bp of the recA gene and of 402 bp of the glnA gene also separated the V. vulnificus population into two major divisions in a manner similar to that by MLEE and RAPD. Sequence data again indicated the overall subdivision of the V. vulnificus population into different biotypes. In particular, indole-negative eel-pathogenic isolates (biotype 2) on one hand and the Israeli isolates (biotype 3) on the other tended to cluster together in both gene trees. None of the methods showed an association between distinct clones and human clinical manifestations. Furthermore, except for the Israeli strains, only minor clusters comprising geographically related isolates were observed. In conclusion, all three approaches (MLEE, RAPD, and DNA sequencing) generated comparable but not always equivalent results. The significance of the two divisions (divisions I and II) still remains to be clarified, and a reevaluation of the definition of the biotypes is also needed
dc.description grants 31-45914.95 and 31-64976.01 from the Swiss National Science Foundation
dc.format 230375 bytes
dc.format 2459 bytes
dc.format application/pdf
dc.format text/plain
dc.language eng
dc.publisher American Society for Microbiology
dc.rights closedAccess
dc.subject Vibrio vulnificus
dc.subject multilocus enzyme electrophoresis
dc.subject random amplification of polymorphic DNA
dc.title Population Genetics of Vibrio vulnificus: Identification of Two Divisions and a Distinct Eel-Pathogenic Clone
dc.type Artículo


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