المستودع الأكاديمي جامعة المدينة

Blocking agents for ELISA quantification of cathepsin L in bovine and porcine muscle crude extracts

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dc.creator Sentandreu, Miguel Angel
dc.creator Aubry, Lorent
dc.creator Ouali, Ahmed
dc.creator Toldrá Vilardell, Fidel
dc.date 2008-02-25T10:36:34Z
dc.date 2008-02-25T10:36:34Z
dc.date 2004-08-08
dc.date.accessioned 2017-01-31T01:00:22Z
dc.date.available 2017-01-31T01:00:22Z
dc.identifier 50th International Congress of Meat Science and Technology. Helsinki, 8-13 August, 2004
dc.identifier http://hdl.handle.net/10261/3053
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3053
dc.description Skimmed milk revealed to be the most efficient of the assayed blockers, as also observed by other authors. In the case of pork, this would be the only suitable blocking agent but avoiding the highest assayed muscle crude extract concentration. All the same attention must be paid, since some authors pointed out the possibility that skimmed milk may interfere the antigen-antibody reaction under certain conditions Vogt et al., 1987. For ELISA quantification of cathepsin L from bovine species, goat serum and BSA, together with skimmed milk, could be used as effective blockers always that a surcoating steps be included in addition to be present in the assay buffer.
dc.description Marie Curie Individual Fellowship (Q1-CT-2002-51527)
dc.format 73469 bytes
dc.format 2459 bytes
dc.format application/pdf
dc.format text/plain
dc.language eng
dc.publisher American Meat Science Association
dc.rights openAccess
dc.subject Cathepsin L
dc.subject ELISA
dc.title Blocking agents for ELISA quantification of cathepsin L in bovine and porcine muscle crude extracts
dc.type Presentación


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