| dc.creator |
Nissen, Lorenzo |
|
| dc.creator |
Yebra, María Jesús |
|
| dc.creator |
Sgobarti, Barbara |
|
| dc.creator |
Pérez Martínez, Gaspar |
|
| dc.date |
2008-02-25T10:36:31Z |
|
| dc.date |
2008-02-25T10:36:31Z |
|
| dc.date |
2005-04 |
|
| dc.date.accessioned |
2017-01-31T01:00:21Z |
|
| dc.date.available |
2017-01-31T01:00:21Z |
|
| dc.identifier |
Biofutura 2005, Bologna, 8-10 Aprile 2005 |
|
| dc.identifier |
http://hdl.handle.net/10261/3051 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3051 |
|
| dc.description |
Sorbitol is a sugar polyol claimed to have health-promoting properties.
D-sorbitol-6-phosphate dehydrogeanse (StolPDh) is required for sorbose and sorbitol metabolism in Lactobacillus casei. StolPDh catalyzes the oxidation of Sorbitol-6-phosphate and also the reverse reaction, or reather, the reduction of fructose-6-phosphate with NAD+ regeneration. In order to test this function in vivo, a new food grade recombinant strain of L. casei was constructed by the integration of a StolPDh-encoding gene (gutF) in the chromosomal lactose operon. Lactose induction of gutF expression was achieved and sorbitol production could be detected by 13C-nuclear magnetic resonance analys, upon addiction of glucose to resting cells. Additional inactivationn of L-lactate dehydrogenase led to higher sorbitol production. These outcomes indicates that the accumulation of the glycolytic precursor, fructose-6-phosphate and an high NAD+/NADH ratio were the limiting factors for sorbitol accumulation. |
|
| dc.format |
213952 bytes |
|
| dc.format |
2459 bytes |
|
| dc.format |
application/pdf |
|
| dc.format |
text/plain |
|
| dc.language |
eng |
|
| dc.publisher |
Associazione Nazionale Biotecnologi Italiani |
|
| dc.rights |
openAccess |
|
| dc.subject |
Lactobacillus casei |
|
| dc.subject |
Sorbitol |
|
| dc.title |
Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei. |
|
| dc.type |
Presentación |
|