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Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide

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dc.creator Solà, Anna M.
dc.creator Roselló-Catafau, Joan
dc.creator Gelpí, Emili
dc.creator Hotter, Georgina
dc.date 2008-02-18T18:19:44Z
dc.date 2008-02-18T18:19:44Z
dc.date 2001-02
dc.date.accessioned 2017-01-31T01:00:11Z
dc.date.available 2017-01-31T01:00:11Z
dc.identifier Gut. 2001 February; 48(2): 168–175.
dc.identifier http://dx.doi.org/10.1136/gut.48.2.168
dc.identifier 0017-5749
dc.identifier http://hdl.handle.net/10261/2972
dc.identifier 10.1136/gut.48.2.168
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2972
dc.description [BACKGROUND AND AIMS] Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by nitric oxide (NO) in intestinal preconditioning could modify the rate of formation of glycolytic intermediates. Fructose-1,6-biphosphate (F16BP) is a glycolytic intermediate that protects tissue from ischaemia/reperfusion injury. We evaluated if F16BP may be endogenously accumulated as a consequence of GAPDH inhibition by NO during intestinal preconditioning in rats.
dc.description [METHODS] We assessed: (1) effect of preconditioning on F16BP content; (2) effect of NO on GAPDH activity before and during sustained ischaemia; and (3) protective effect of F16BP in control, ischaemic, and preconditioned animals with or without administration of N-nitro-L-arginine methyl ester (L-NAME), NO donor, or F16BP.
dc.description [RESULTS] Preconditioned rats showed a significant transient decrease in GAPDH activity and also maintained basal F16BP levels longer than ischaemic rats. L-NAME administration to preconditioned rats reversed these effects. F16BP administration to ischaemic rats decreased protein release in the perfusate. Administration of F16BP to L-NAME treated rats attenuated the harmful effect of L-NAME.
dc.description [CONCLUSIONS] Our study indicates that F16BP may be endogenously accumulated in preconditioned rats as a consequence of inhibition of GAPDH by NO, and this may contribute to the protection observed in intestinal preconditioning.
dc.description This work was supported by grant FIS 98/002901. A Sola was supported by a grant from Institut d’Investigacions Biomèdiques August Pi I Sunyer.
dc.description Peer reviewed
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dc.language eng
dc.publisher BMJ Publishing Group
dc.relation http://dx.doi.org/10.1136/gut.48.2.168
dc.rights openAccess
dc.subject Fructose-1,6-biphosphate
dc.subject Glyceraldehyde- 3-phosphate dehydrogenase
dc.subject Intestinal preconditioning
dc.subject Ischaemia/reperfusion injury
dc.subject Nitric Oxide
dc.title Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide
dc.type Artículo


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