المستودع الأكاديمي جامعة المدينة

In vivo antioxidant treatment protects against bleomycin-induced lung damage in rats

أعرض تسجيلة المادة بشكل مبسط

dc.creator Serrano-Mollar, Anna
dc.creator Closa, Daniel
dc.creator Morcillo, Esteban J.
dc.creator Bulbena, Oriol
dc.date 2008-02-18T18:03:08Z
dc.date 2008-02-18T18:03:08Z
dc.date 2003-03-26
dc.date.accessioned 2017-01-31T01:00:10Z
dc.date.available 2017-01-31T01:00:10Z
dc.identifier British Journal of Pharmacology 138(6): 1037–1048 (2003)
dc.identifier 0007-1188
dc.identifier http://hdl.handle.net/10261/2971
dc.identifier 10.1038/sj.bjp.0705138
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2971
dc.description This study examines the activity of the antioxidant N-acetylcysteine on bleomycin-induced pulmonary fibrosis in rats with emphasis on the early inflammatory phase. Rats receiving N-acetylcysteine (300 mg kg−1 day−1, intraperitoneal) had less augmented lung wet weight, and lower levels of proteins, lactate dehydrogenase, neutrophil and macrophage counts in bronchoalveolar lavage fluid and lung myeloperoxidase activity with a betterment of histological score at 3 days postbleomycin. A diminished lung GSH/GSSG ratio and augmented lipid hydroperoxides were observed 3 days postbleomycin. These changes were attenuated by N-acetylcysteine. Alveolar macrophages from bleomycin-exposed rats released augmented amounts of superoxide anion and nitric oxide. N-Acetylcysteine did not modify superoxide anion generation but reduced the increased production of nitric oxide. N-Acetylcysteine suppressed the bleomycin-induced increased activation of lung NF-κB (shift assay and immunohistochemistry), and decreased the augmented levels of the early inflammatory cytokines, tumour necrosis factor-α, interleukin-β, interleukin-6 and macrophage inflammatory protein-2 observed in bronchoalveolar lavage fluid at 1 and 3 days postbleomycin exposure. At 15 days postbleomycin, N-acetylcysteine decreased collagen deposition in bleomycin-exposed rats (hydroxyproline content: 6351±669 and 4626±288 μg per lung in drug vehicle- and N-acetylcysteine-treated rats, respectively; P<0.05). Semiquantitative histological assessment at this stage showed less collagen deposition in N-acetylcysteine-treated rats compared to those receiving bleomycin alone. These results indicate that N-acetylcysteine reduces the primary inflammatory events, thus preventing cellular damage and the subsequent development of pulmonary fibrosis in the bleomycin rat model.
dc.description This work was supported by Grant 1FD97-1143 from the European Union (Regional Development Funds, FEDER), CICYT (Spanish Government), Regional Government (Generalitat Valenciana) and Grant FIS98/1367 (Spanish Ministry of Health).
dc.description Peer reviewed
dc.format 481474 bytes
dc.format application/pdf
dc.language eng
dc.publisher Wiley-Blackwell
dc.relation http://dx.doi.org/10.1038/sj.bjp.0705138
dc.rights openAccess
dc.subject Pulmonary fibrosis
dc.subject Bleomycin
dc.subject Rat
dc.subject N-acetylcysteine
dc.subject Inflammation
dc.title In vivo antioxidant treatment protects against bleomycin-induced lung damage in rats
dc.type Artículo


الملفات في هذه المادة

الملفات الحجم الصيغة عرض

لا توجد أي ملفات مرتبطة بهذه المادة.

هذه المادة تبدو في المجموعات التالية:

أعرض تسجيلة المادة بشكل مبسط