المستودع الأكاديمي جامعة المدينة

Changes in photosynthetic electron transfer and state transitions in an herbicide-resistant D1 mutant from soybean cell cultures

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dc.creator Roncel Gil, Mercedes
dc.creator Yruela Guerrero, Inmaculada
dc.creator Kirilovsky, D.
dc.creator Guerrero, Fernando
dc.creator Alfonso Lozano, Miguel
dc.creator Picorel Castaño, Rafael
dc.creator Ortega, José M.
dc.date 2008-02-11T13:24:52Z
dc.date 2008-02-11T13:24:52Z
dc.date 2007-06
dc.date.accessioned 2017-01-31T01:00:06Z
dc.date.available 2017-01-31T01:00:06Z
dc.identifier Biochimica et Biophysica Acta (BBA) - Bioenergetics 1767 (2007) 694-702.
dc.identifier 0005-2728
dc.identifier http://hdl.handle.net/10261/2917
dc.identifier 10.1016/j.bbabio.2007.02.017
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2917
dc.description The definitive version is available at: http://www.sciencedirect.com/science//journal/00052728
dc.description Anomalies in photosynthetic activity of the soybean cell line STR7, carrying a single mutation (S268P) in the chloroplastic gene psbA that codes for the D1 protein of the photosystem II, have been examined using different spectroscopic techniques. Thermoluminescence emission experiments have shown important differences between STR7 mutant and wild type cells. The afterglow band induced by both white light flashes and far-red continuous illumination was downshifted by about 4 °C and the Q band was upshifted by 5 °C. High temperature thermoluminescence measurements suggested a higher level of lipid peroxidation in mutant thylakoid membranes. In addition, the reduction rate of P700+ was significantly accelerated in STR7 suggesting that the mutation led to an activation of the photosystem I cyclic electron flow. Modulated fluorescence measurements performed at room temperature as well as fluorescence emission spectra at 77 K revealed that the STR7 mutant is defective in state transitions. Here, we discuss the hypothesis that activation of the cyclic electron flow in STR7 cells may be a mechanism to compensate the reduced activity of photosystem II caused by the mutation. We also propose that the impaired state transitions in the STR7 cells may be due to alterations in thylakoid membrane properties induced by a low content of unsaturated lipids.
dc.description This work was supported by grants from the Ministry of Education and Culture of Spain (BFU-BMC2004-04914-C02-01, BMC2002-00031 and BFU-BMC2005-07422-C02-01) and Andalusia Government (PAI CVI-261).
dc.description Peer reviewed
dc.format 321411 bytes
dc.format application/pdf
dc.language eng
dc.publisher Elsevier
dc.rights openAccess
dc.subject Cyclic and linear electron flow
dc.subject Photosystem I
dc.subject Photosystem II
dc.subject Thermoluminiscence
dc.subject State transitions
dc.subject D1 mutant
dc.title Changes in photosynthetic electron transfer and state transitions in an herbicide-resistant D1 mutant from soybean cell cultures
dc.type Artículo


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