| dc.creator |
María, Nuria de |
|
| dc.creator |
Guevara, Angeles |
|
| dc.creator |
Serra, María Teresa |
|
| dc.creator |
García-Luque, Isabel |
|
| dc.creator |
González-Sama, Alfonso |
|
| dc.creator |
García de Lacoba, Mario |
|
| dc.creator |
Felipe, Mª Rosario de |
|
| dc.creator |
Fernández-Pascual, Mercedes |
|
| dc.date |
2008-02-04T18:48:48Z |
|
| dc.date |
2008-02-04T18:48:48Z |
|
| dc.date |
2007-06-08 |
|
| dc.date.accessioned |
2017-01-31T00:59:56Z |
|
| dc.date.available |
2017-01-31T00:59:56Z |
|
| dc.identifier |
Applied and Environmental Microbiology: 73(16): 5075–5082 (2007) |
|
| dc.identifier |
0099-2240 |
|
| dc.identifier |
http://hdl.handle.net/10261/2841 |
|
| dc.identifier |
10.1128/AEM.00392-07 |
|
| dc.identifier |
1098-5336 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2841 |
|
| dc.description |
Application of glyphosate (N-[phosphonomethyl] glycine) to Bradyrhizobium sp. (Lupinus)-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. (Lupinus) putative porin, and would be the first porin described in Bradyrhizobium sp. (Lupinus). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed. |
|
| dc.description |
This work was supported by grants 142/CH-39 and 168/CH-47 from Junta de Comunidades de Castilla-La Mancha (C-LM) and BIO2004-04968-C02 from CICYT. N.D.M. was supported by a C-LM predoctoral fellowship. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
635775 bytes |
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| dc.format |
application/pdf |
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| dc.language |
eng |
|
| dc.publisher |
American Society for Microbiology |
|
| dc.relation |
http://dx.doi.org/10.1128/AEM.00392-07 |
|
| dc.rights |
openAccess |
|
| dc.title |
Putative Porin of Bradyrhizobium sp. (Lupinus) Bacteroids Induced by Glyphosate |
|
| dc.type |
Artículo |
|