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Method for extracting gluten contained in heat-processed and non-heat-processed foodstuffs, compatible with an enzyme-linked immunosorbent assay, composition and kits comprising said composition

Method for extracting gluten contained in heat-processed and non-heat-processed foodstuffs, compatible with an enzyme-linked immunosorbent assay, composition and kits comprising said composition

Title: Method for extracting gluten contained in heat-processed and non-heat-processed foodstuffs, compatible with an enzyme-linked immunosorbent assay, composition and kits comprising said composition;
Procedimiento para la extracción de gluten en alimentos, procesados y no procesados por calor, compatible con ELISA, composición y kits que comprenden dicha composición
López Villar, Elena; Llorente Gómez, María de las Mercedes; Méndez Cormán, Enrique
URI: http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2706

Description:

 
Filing Date: 2002-05-03.-- Priority Data: ES (2001-05-14)P- (2001-01-09)
 
The invention relates to a method for extracting the gluten contained in a sample consisting in using an ethanol aqueous solution in the presence of a composition containing a disulphur group reducer and a decoupling agent, in a buffer having a pH of between 7 and 8. The inventive method can be used to extract quantitatively the gluten contained in a heat-processed or non-heat-processed food sample before the quantification of the gluten by ELISA. Said method is suitable for food analyses, in particular, for foodstuffs intended for coeliac sufferers.
 
Peer reviewed
 

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