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Method for the immobilisation of biomacromolecules on supports that are activated with epoxide groups

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dc.creator Guisán, José Manuel
dc.creator Fernández-Lafuente, Roberto
dc.creator Mateo González, César
dc.creator Torres Sáez, Rodrigo
dc.creator Fernández-Lorente, Gloria
dc.creator Ortiz, Claudia
dc.creator Fuentes García, Manuel
dc.creator Hidalgo, Aurelio
dc.creator Palomo, José Miguel
dc.creator López Gallego, Fernando
dc.creator Betancor, Lorena
dc.creator Pessela, Benevides C.
dc.date 2008-01-17T16:41:22Z
dc.date 2008-01-17T16:41:22Z
dc.date 2004-09-02
dc.date.accessioned 2017-01-31T00:59:43Z
dc.date.available 2017-01-31T00:59:43Z
dc.identifier International Publication Number: WO 2004/074471 A1
dc.identifier http://hdl.handle.net/10261/2701
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2701
dc.description Filing Date: 2004-02-20.--Priority Data ES P200300428 (2003-02-21)
dc.description The invention relate to a method for the immobilisation of biomacromolecules on supports that are activated with epoxide groups.More specifically, the invention relates to a method for the immobilisation of enzymes and proteins on said type of support, which comprises two consecutive steps, namely: (a) very rapid ionic absorption of the enzyme on the support which is ionised under very gentle experimental conditions; and (b) intense multipoint covalent immobilisation between the previously absorbed enzyme and a very high density of epoxide groups present in the support. The main practical characteristics of said novel immobilisation method are: the very rapid immobilisation of large quantities of enzyme under very gentle experimental conditions, the possibility of stabilising the enzymes immobilised by means of multipoint covalent immobilisation to the support and the fact that no unstable reagent or toxic material is used during or after the immobilisation, etc.
dc.format 1076416 bytes
dc.format application/pdf
dc.language spa
dc.rights openAccess
dc.title Method for the immobilisation of biomacromolecules on supports that are activated with epoxide groups
dc.type Patente


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