| dc.creator |
Cañada Vicinay, Francisco Javier |
|
| dc.creator |
Corrales, Guillermo |
|
| dc.creator |
Fernández-Mayoralas, Alfonso |
|
| dc.creator |
Martín-Lomas, Manuel |
|
| dc.creator |
Aragón, Juan J. |
|
| dc.date |
2008-01-16T17:33:39Z |
|
| dc.date |
2008-01-16T17:33:39Z |
|
| dc.date |
2006-03-29 |
|
| dc.date.accessioned |
2017-01-31T00:59:43Z |
|
| dc.date.available |
2017-01-31T00:59:43Z |
|
| dc.identifier |
European Patent Code: EP 1408118 B1 |
|
| dc.identifier |
http://hdl.handle.net/10261/2698 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2698 |
|
| dc.description |
Filing Date: 2002-06-14.-- Priority Data: ES 200101419 (2001-06-18).-- International Publication Number: WO_2002103038 (20021227). |
|
| dc.description |
An enzymatic process to obtain 4-O-β-D-galactopyranosyl-D-xylose useful in compositions or solutions in the in vivo evaluation of intestinal lactose activity in humans, that comprises the steps of preparing a reaction mixture of D-xylose, a β-D-galactopyranoside and a reaction medium that comprises water buffered to a pH between 5.0 and 9.0; adding 10 to 1,000 units of β-D-galactosidase per gram of β-D-galactopyranoside; subjecting the reaction mixture to a reaction or a temperature between a temperature higher than the freezing point of the reaction mixture and 45ºC, for 2 to 48 hours; for the reaction by deactivation of the β-D-galactosidase; and to isolate and crystallize the fractions that contain 4-O-β-D-galactopyranosyl-D-xylose from a crystallization mixture selected between mixtures of acetone/methanol in a ratio between 5/1 to 20/1 and mixtures of acetone/water in a ratio between 5/1 to 20/1. |
|
| dc.format |
185663 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.rights |
openAccess |
|
| dc.title |
Enzymatic method of producing 4-O- β-D-galactopyranosyl-D-xylose, 4-O- β-D-galactopyranosyl-D-xylose obtained using said method, compositions containing said and the use thereof in evaluating intestinal lactase |
|
| dc.type |
Patente |
|