| dc.creator |
Montañez Sáenz, Julio |
|
| dc.creator |
Téllez, Alejandro |
|
| dc.creator |
Garza, Heliodoro de la |
|
| dc.creator |
Reyes, María de la Luz |
|
| dc.creator |
Contreras-Esquivel, Juan Carlos |
|
| dc.creator |
Aguilar, Cristóbal Noé |
|
| dc.date |
2007-11-15T15:50:16Z |
|
| dc.date |
2007-11-15T15:50:16Z |
|
| dc.date |
2000 |
|
| dc.date.accessioned |
2017-01-31T00:58:41Z |
|
| dc.date.available |
2017-01-31T00:58:41Z |
|
| dc.identifier |
Braz. arch. biol. technol., 2000, vol.43, no.4, p.0-0 |
|
| dc.identifier |
1516-8913 |
|
| dc.identifier |
http://hdl.handle.net/10261/2173 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2173 |
|
| dc.description |
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel |
|
| dc.description |
Authors wish to thank National Council for Science & Technology (CONACYT, México 83076/124550). This research was partially supported by the CGEPI-UAdeC and forms part of the thesis work of Julio Cesar Montañez. Authors wish to thank researchers of PP-4 of Biotechnology Dept, in the Universidad Autonoma Metropolitana Iztapalapa for facilities and critical opinions offered. |
|
| dc.description |
Peer reviewed |
|
| dc.language |
eng |
|
| dc.publisher |
Instituto de Tecnologia do Paraná |
|
| dc.rights |
openAccess |
|
| dc.subject |
Extraction |
|
| dc.subject |
Purification |
|
| dc.subject |
Properties |
|
| dc.subject |
Pectinesterase |
|
| dc.subject |
Potato |
|
| dc.title |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
|
| dc.type |
Artículo |
|