أعرض تسجيلة المادة بشكل مبسط
| dc.creator |
Rentero, Carles |
|
| dc.creator |
Puigdomènech, Pere |
|
| dc.date |
2007-05-08T16:06:17Z |
|
| dc.date |
2007-05-08T16:06:17Z |
|
| dc.date |
2006-11-08 |
|
| dc.date.accessioned |
2017-01-31T00:57:13Z |
|
| dc.date.available |
2017-01-31T00:57:13Z |
|
| dc.identifier |
BMC Molecular Biology 2006, 7:39 |
|
| dc.identifier |
1471-2199 |
|
| dc.identifier |
http://hdl.handle.net/10261/1442 |
|
| dc.identifier |
10.1186/1471-2199-7-39 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/1442 |
|
| dc.description |
[Background] Phosphodiesterases are an important protein family that catalyse the hydrolysis of
cyclic nucleotide monophosphates (cAMP and cGMP), second intracellular messengers responsible
for transducing a variety of extra-cellular signals. A number of different splice variants have been
observed for the human phosphodiesterase 9A gene, a cGMP-specific high-affinity PDE. These
mRNAs differ in the use of specific combinations of exons located at the 5' end of the gene while
the 3' half, that codes for the catalytic domain of the protein, always has the same combination of
exons. It was observed that to deduce the protein sequence with the catalytic domain from all the
variants, at least two ATG start codons have to be used. Alternatively some variants code for
shorter non-functional polypeptides. |
|
| dc.description |
[Results] In the present study, we expressed different splice variants of PDE9A in HeLa and Cos-
1 cells with EGFP fluorescent protein in phase with the catalytic domain sequence in order to test
the different start codon usage in each splice variant. It was found that at least two ATG start
codons may be used and that the open reading frame that includes the catalytic domain may be
translated. In addition the proteins produced from some of the splice variants are targeted to
membrane ruffles and cellular vesicles while other variants appear to be cytoplasmic. A hypothesis
about the functional meaning of these results is discussed. |
|
| dc.description |
[Conclusion] Our data suggest the utilization of two different start codons to produce a variety of
different PDE9A proteins, allowing specific subcellular location of PDE9A splice variants. |
|
| dc.description |
Peer reviewed |
|
| dc.language |
eng |
|
| dc.publisher |
BioMed Central |
|
| dc.relation |
Publisher’s version |
|
| dc.rights |
openAccess |
|
| dc.title |
Specific use of start codons and cellular localization of splice variants of human phosphodiesterase 9A gene |
|
| dc.type |
Artículo |
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أعرض تسجيلة المادة بشكل مبسط