المستودع الأكاديمي جامعة المدينة

Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma

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dc.creator Lázcoz, Paula
dc.creator Muñoz, Jorge
dc.creator Nistal, Manuel
dc.creator Pestaña, Ángel
dc.creator Encío, Ignacio
dc.creator Castresana, Javier S.
dc.date 2007-05-08T12:43:26Z
dc.date 2007-05-08T12:43:26Z
dc.date 2006-10-25
dc.date.accessioned 2017-01-31T00:57:09Z
dc.date.available 2017-01-31T00:57:09Z
dc.identifier BMC Cancer 2006, 6: 254 (2006)
dc.identifier 1471-2407
dc.identifier http://hdl.handle.net/10261/1413
dc.identifier 10.1186/1471-2407-6-254
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/1413
dc.description [Background] Epigenetic alterations and loss of heterozygosity are mechanisms of tumor suppressor gene inactivation. A new carcinogenic pathway, targeting the RAS effectors has recently been documented. RASSF1A, on 3p21.3, and NORE1A, on 1q32.1, are among the most important, representative RAS effectors.
dc.description [Methods] We screened the 3p21 locus for the loss of heterozygosity and the hypermethylation status of RASSF1A, NORE1A and BLU (the latter located at 3p21.3) in 41 neuroblastic tumors. The statistical relationship of these data was correlated with CASP8 hypermethylation. The expression levels of these genes, in cell lines, were analyzed by RT-PCR.
dc.description [Results] Loss of heterozygosity and microsatellite instability at 3p21 were detected in 14% of the analyzed tumors. Methylation was different for tumors and cell lines (tumors: 83% in RASSF1A, 3% in NORE1A, 8% in BLU and 60% in CASP8; cell lines: 100% in RASSF1A, 50% in NORE1A, 66% in BLU and 92% in CASP8). In cell lines, a correlation with lack of expression was evident for RASSF1A, but less clear for NORE1A, BLU and CASP8. We could only demonstrate a statistically significant association between hypermethylation of RASSF1A and hypermethylation of CASP8, while no association with MYCN amplification, 1p deletion, and/or aggressive histological pattern of the tumor was demonstrated.
dc.description [Conclusion] 1) LOH at 3p21 appears in a small percentage of neuroblastomas, indicating that a candidate tumor suppressor gene of neuroblastic tumors is not located in this region. 2) Promoter hypermethylation of RASSF1A and CASP8 occurs at a high frequency in neuroblastomas.
dc.description Peer reviewed
dc.language eng
dc.publisher BioMed Central
dc.relation Publisher’s version
dc.relation http://dx.doi.org/10.1186/1471-2407-6-254
dc.rights openAccess
dc.title Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma
dc.type Artículo


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