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Computational and experimental analysis identifies Arabidopsis genes specifically expressed during early seed development

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dc.creator Becerra, Cristian
dc.creator Puigdomènech, Pere
dc.creator Vicient, Carlos M
dc.date 2007-05-08T12:10:02Z
dc.date 2007-05-08T12:10:02Z
dc.date 2006-02-28
dc.date.accessioned 2017-01-31T00:57:08Z
dc.date.available 2017-01-31T00:57:08Z
dc.identifier BMC Genomics 7(38): 2006
dc.identifier http://dx.doi.org/10.1186/1471-2164-7-38
dc.identifier 1471-2164
dc.identifier http://hdl.handle.net/10261/1406
dc.identifier 10.1186/1471-2164-7-38
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/1406
dc.description [Background] Plant seeds are complex organs in which maternal tissues, embryo and endosperm, follow distinct but coordinated developmental programs. Some morphogenetic and metabolic processes are exclusively associated with seed development. The goal of this study was to explore the feasibility of incorporating the available online bioinformatics databases to discover Arabidopsis genes specifically expressed in certain organs, in our case immature seeds.
dc.description [Results] A total of 11,032 EST sequences obtained from isolated immature seeds were used as the initial dataset (178 of them newly described here). A pilot study was performed using EST virtual subtraction followed by microarray data analysis, using the Genevestigator tool. These techniques led to the identification of 49 immature seed-specific genes. The findings were validated by RT-PCR analysis and in situ hybridization.
dc.description [Conclusion] We conclude that the combined in silico data analysis is an effective data mining strategy for the identification of tissue-specific gene expression.
dc.description Peer reviewed
dc.language eng
dc.publisher BioMed Central
dc.relation Publisher’s version
dc.rights openAccess
dc.rights http://creativecommons.org/licenses/by/2.0/
dc.title Computational and experimental analysis identifies Arabidopsis genes specifically expressed during early seed development
dc.type Artículo


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