| dc.contributor |
Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, Spain. Jose.Ainsa@msf.unizar.es |
|
| dc.creator |
Aínsa, J |
|
| dc.creator |
Martin, C |
|
| dc.creator |
Gicquel, B |
|
| dc.creator |
Gomez-Lus, R |
|
| dc.date |
1996-10 |
|
| dc.date.accessioned |
2017-01-31T07:10:03Z |
|
| dc.date.available |
2017-01-31T07:10:03Z |
|
| dc.identifier |
Characterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum. 1996, 40 (10):2350-5 Antimicrob. Agents Chemother. |
|
| dc.identifier |
0066-4804 |
|
| dc.identifier |
8891143 |
|
| dc.identifier |
http://hdl.handle.net/10144/18395 |
|
| dc.identifier |
http://fieldresearch.msf.org/msf/handle/10144/18395 |
|
| dc.identifier |
Antimicrobial Agents and Chemotherapy |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10144/18395 |
|
| dc.description |
A novel gene encoding an aminoglycoside 2'-N-acetyltransferase (AAC) was cloned from Mycobacterium fortuitum. DNA sequencing results identified an open reading frame that we have called aac(2')-Ib encoding a putative protein with a predicted molecular mass of 24,800 Da. The deduced AAC(2')-Ib protein showed homology to the AAC(2')-Ia from Providencia stuartii. This is the second member of a subfamily of AAC(2')-I enzymes to be identified. No homology was found with other acetyltransferases, including all of the AAC(3) and AAC(6') proteins. The aac(2')-Ib gene cloned in a mycobacterial plasmid and introduced in Mycobacterium smegmatis conferred resistance to gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin. DNA hybridization with an intragenic probe of aac(2')-Ib showed that this gene was present in all 34 strains of M. fortuitum tested. The universal presence of the aac(2')-Ib gene in M. fortuitum was not correlated with any aminoglycoside resistance phenotype, suggesting that this gene may play a role in the secondary metabolism of the bacterium. |
|
| dc.language |
en |
|
| dc.publisher |
Published by American Society for Microbiology |
|
| dc.rights |
Archived on this site with permission and copyright by the American Society for Microbiology |
|
| dc.title |
Characterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum. |
|