| dc.contributor |
Médecins Sans Frontières (MSF) - Switzerland, Geneva, Switzerland. francois.chappuis@hcuge.ch |
|
| dc.creator |
Chappuis, F |
|
| dc.creator |
Pittet, A |
|
| dc.creator |
Bovier, P A |
|
| dc.creator |
Adams, K |
|
| dc.creator |
Godineau, V |
|
| dc.creator |
Hwang, S Y |
|
| dc.creator |
Magnus, E |
|
| dc.creator |
Büscher, P |
|
| dc.date |
2002-11 |
|
| dc.date.accessioned |
2017-01-31T07:09:12Z |
|
| dc.date.available |
2017-01-31T07:09:12Z |
|
| dc.identifier |
Field evaluation of the CATT/Trypanosoma brucei gambiense on blood-impregnated filter papers for diagnosis of human African trypanosomiasis in southern Sudan. 2002, 7 (11):942-8 Trop. Med. Int. Health |
|
| dc.identifier |
1360-2276 |
|
| dc.identifier |
12390600 |
|
| dc.identifier |
http://hdl.handle.net/10144/17295 |
|
| dc.identifier |
http://fieldresearch.msf.org/msf/handle/10144/17295 |
|
| dc.identifier |
Tropical Medicine & International Health |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10144/17295 |
|
| dc.description |
Most Human African Trypanosomiasis (HAT) control programmes in areas endemic for Trypanosoma brucei gambiense rely on a strategy of active mass screening with the Card Agglutination Test for Trypanosomiasis (CATT)/T. b. gambiense. We evaluated the performance, stability and reproducibility of the CATT/T. b. gambiense on blood-impregnated filter papers (CATT-FP) in Kajo-Keji County, South-Sudan, where some areas are inaccessible to mobile teams. The CATT-FP was performed with a group of 100 people with a positive CATT on whole blood including 17 confirmed HAT patients and the results were compared with the CATT on plasma (CATT-P). The CATT-FP was repeated on impregnated filter papers stored at ambient and refrigerated temperature for 1, 3, 7 and 14 days. Another 82 patients with HAT, including 78 with a positive parasitology, were tested with the CATT-FP and duplicate filter paper samples were sent to a reference laboratory to assess reproducibility. The CATT-FP was positive in 90 of 99 patients with HAT (sensitivity: 91%). It was less sensitive than the CATT-P (mean dilution difference: -2.5). There was no significant loss of sensitivity after storage for up to 14 days both at ambient and cool temperature. Reproducibility of the CATT-FP was found to be excellent (kappa: 0.84). The CATT-FP can therefore be recommended as a screening test for HAT in areas where the use of CATT-P is not possible. Further studies on larger population samples in different endemic foci are still needed before the CATT-FP can be recommended for universal use. |
|
| dc.language |
en |
|
| dc.publisher |
Wiley-Blackwell |
|
| dc.relation |
http://www.blackwell-synergy.com/loi/tmi |
|
| dc.rights |
Archived on this site with the kind permission of Wiley-Blackwell |
|
| dc.title |
Field evaluation of the CATT/Trypanosoma brucei gambiense on blood-impregnated filter papers for diagnosis of human African trypanosomiasis in southern Sudan. |
|