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http://dspace.mediu.edu.my:8181/xmlui/handle/2431/73Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Manzanares, Paloma | - |
| dc.creator | van den Broeck, Hetty C. | - |
| dc.creator | Graaff, Leo H. de | - |
| dc.creator | Visser, Jaap | - |
| dc.date | 2008-02-25T10:40:31Z | - |
| dc.date | 2008-02-25T10:40:31Z | - |
| dc.date | 2001-05 | - |
| dc.date.accessioned | 2017-01-31T01:00:23Z | - |
| dc.date.available | 2017-01-31T01:00:23Z | - |
| dc.identifier | Applied and Environmental Microbiology 67(5) : 2230-2234 (2001) | - |
| dc.identifier | http://hdl.handle.net/2431/73 | - |
| dc.identifier | http://hdl.handle.net/10261/3070 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/2431/73 | - |
| dc.description | Two proteins exhibiting -L-rhamnosidase activity, RhaA and RhaB, were identified upon fractionation and purification of a culture filtrate from Aspergillus aculeatus grown on hesperidin. Both proteins were shown to be N glycosylated and had molecular masses of 92 and 85 kDa, of which approximately 24 and 15%, respectively, were contributed by carbohydrate. RhaA and RhaB, optimally active at pH 4.5 to 5, showed Km and Vmax values of 2.8 mM and 24 U/mg (RhaA) and 0.30 mM and 14 U/mg (RhaB) when tested for p-nitrophenyl--L-rhamnopyranoside. Both enzymes were able to hydrolyze -1,2 and -1,6 linkages to -D-glucosides. Using polyclonal antibodies, the corresponding cDNA of both -L-rhamnosidases, rhaA and rhaB, was cloned. On the basis of the amino acid sequences derived from the cDNA clones, both proteins are highly homologous (60% identity). | - |
| dc.description | EC project AIR3-CT94-2193 | - |
| dc.format | 141808 bytes | - |
| dc.format | 2459 bytes | - |
| dc.format | application/pdf | - |
| dc.format | text/plain | - |
| dc.language | eng | - |
| dc.publisher | American Society for Microbiology | - |
| dc.rights | closedAccess | - |
| dc.subject | Aspergillus aculeatus | - |
| dc.subject | Rhamnosidases | - |
| dc.title | Purification and Characterization of Two Different -L-Rhamnosidases,RhaA and RhaB, from Aspergillus aculeatus | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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