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http://dspace.mediu.edu.my:8181/xmlui/handle/1721.1/3784Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Yuk, Inn Huam Yvonne. | - |
| dc.creator | Wang, Daniel I.C. | - |
| dc.date | 2003-12-08T15:24:37Z | - |
| dc.date | 2003-12-08T15:24:37Z | - |
| dc.date | 2003-01 | - |
| dc.date.accessioned | 2013-10-09T02:32:27Z | - |
| dc.date.available | 2013-10-09T02:32:27Z | - |
| dc.date.issued | 2013-10-09 | - |
| dc.identifier | http://hdl.handle.net/1721.1/3784 | - |
| dc.identifier.uri | http://koha.mediu.edu.my:8181/xmlui/handle/1721 | - |
| dc.description | N-linked glycosylation often imparts important properties to protein therapeutics. An essential step in this intracellular process is the transfer of oligosaccharide from dolichol monophosphate (Dol-P) to a potential glycosylation site. Variability in the success rate of this reaction affects the extent of protein glycosylation. The critical role of Dol-P suggests that its availability may influence the extent of glycosylation by limiting the pool of lipid-linked oligosaccharides (LLOs), the glycosyl donor. To test this hypothesis, the impact of Dol-P supplementation on protein glycosylation in Chinese hamster ovary (CHO) cells was investigated. Although exogenous Dol-P was incorporated by CHO cells and processed into LLOs in a dose-dependent manner, Dol-P supplementation had no marked effects on LLO or overall cellular glycosylation levels. While concentrations of exogenous Dol-P exceeding 100 µg/ml were detrimental to CHO cell viability, maximum non-toxic supplemental doses of Dol-P had no significant impact on the glycosylation of recombinant interferon-γ produced by batch cultures of CHO cells. These results show that glycosylation in CHO cells cannot be readily enhanced by Dol-P feeding under normal culture conditions. | - |
| dc.description | Singapore-MIT Alliance (SMA) | - |
| dc.format | 9846221 bytes | - |
| dc.format | application/pdf | - |
| dc.language | en_US | - |
| dc.relation | Molecular Engineering of Biological and Chemical Systems (MEBCS); | - |
| dc.subject | batch culture | - |
| dc.subject | interferon-γ | - |
| dc.subject | lipid-linked oligosaccharide | - |
| dc.title | Glycosylation by Chinese Hamster Ovary Cells in Dolichol Phosphate-Supplemented Cultures | - |
| dc.type | Article | - |
| Appears in Collections: | MIT Items | |
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