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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4096| Title: | Impairment of transcription elongation by R-loops in vitro |
| Keywords: | Saccharomyces cerevisiae R-loops Transcription elongation In vitro transcription RNA polymerase II DNA–RNA hybrid RNA |
| Publisher: | Elsevier |
| Description: | PMID: 17603014.-- Final version of the paper available at: http://www.sciencedirect.com/science/journal/0006291X Transcription elongation causes a local change in DNA superhelicity. An excess of negative supercoiling may lead to opening of DNA strands that could allow formation of R-loops. In yeast, mutants of the THO complex are impaired in transcription elongation and this defect has been linked to co-transcriptional formation of R-loops, which could constitute roadblocks for RNA polymerases. In this study, we found that stably formed 300-nt long DNA–RNA hybrids in a negatively supercoiled transcription template reduced the efficiency of transcription elongation by half, providing a first experimental evidence that transcription elongation is impaired by R-loops in vitro. This work was supported by grants of the Spanish Ministry of Science and Education (GEN2001-4707-C08-02 and SAF2003-00204) and Junta de Andalucía (CVI102). Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4096 |
| Other Identifiers: | Biochem Biophys Res Commun. 360(2): 428-432 (2007). 0006-291X http://hdl.handle.net/10261/4096 10.1016/j.bbrc.2007.06.098 |
| Appears in Collections: | Digital Csic |
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