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dc.creatorMuñoz-Amatriaín, María-
dc.creatorSvensson, J. T.-
dc.creatorCastillo Alonso, Ana María-
dc.creatorCistué Sola, Luis-
dc.creatorClose, T. J.-
dc.creatorVallés Brau, María Pilar-
dc.date2008-04-18T11:25:52Z-
dc.date2008-04-18T11:25:52Z-
dc.date2006-08-
dc.date.accessioned2017-01-31T01:02:29Z-
dc.date.available2017-01-31T01:02:29Z-
dc.identifierPhysiologia Plantarum 127 (4) , 551–560-
dc.identifier0031-9317-
dc.identifierhttp://hdl.handle.net/10261/3643-
dc.identifier10.1111/j.1399-3054.2006.00729.x-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/3643-
dc.descriptionThe definitive version is available at: http://www.blackwell-synergy.com/doi/full/10.1111/j.1399-3054.2006.00729.x-
dc.descriptionCarbohydrate starvation is an efficient stress treatment for induction of microspore embryogenesis. Transcriptome analysis of anthers response to mannitol treatment using the 22k Barley1 GeneChip revealed large changes in gene expression. Statistical analysis and filtering for 4-fold or greater changes resulted in 2,673 genes, of which 887 were up-regulated and 1,786 down-regulated. Great differences in some metabolic pathways, accompanied by a multi-dimensional stress response were found. Analysis of transcription factors showed that most of the down-regulated transcription factors were related to growth and development, and the up-regulated with abiotic and biotic stress responses and changes in developmental programs. Interestingly, the expression of most cell cycle related genes did not change significantly. Transcriptome analysis provided a successful approach to identify genes involved in mannitol treatment, essential for triggering microspore embryogenesis.-
dc.descriptionMaría Muñoz-Amatriaín is recipient of a FPI fellowship and a Short-term Scientific Mission to UCR, from Ministerio de Educación y Ciencia of Spain. The research was supported by Projects AGL-2001-1631 and AGL-2004-03396, from Plan Nacional I+D+I, Ministerio de Educación y Ciencia of Spain.-
dc.descriptionPeer reviewed-
dc.format150555 bytes-
dc.format12475 bytes-
dc.format128008 bytes-
dc.format76872 bytes-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherBlackwell Publishing-
dc.rightsopenAccess-
dc.subjectBarley-
dc.subjectMicroarray-
dc.subjectMicrospore embryogenesis-
dc.subjectMannitol pretreatment-
dc.subjectTranscription factors-
dc.titleTranscriptome analysis of barley anthers: effect of mannitol treatment on microspore embryogenesis-
dc.typeArtículo-
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