Please use this identifier to cite or link to this item:
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3643| Title: | Transcriptome analysis of barley anthers: effect of mannitol treatment on microspore embryogenesis |
| Keywords: | Barley Microarray Microspore embryogenesis Mannitol pretreatment Transcription factors |
| Publisher: | Blackwell Publishing |
| Description: | The definitive version is available at: http://www.blackwell-synergy.com/doi/full/10.1111/j.1399-3054.2006.00729.x Carbohydrate starvation is an efficient stress treatment for induction of microspore embryogenesis. Transcriptome analysis of anthers response to mannitol treatment using the 22k Barley1 GeneChip revealed large changes in gene expression. Statistical analysis and filtering for 4-fold or greater changes resulted in 2,673 genes, of which 887 were up-regulated and 1,786 down-regulated. Great differences in some metabolic pathways, accompanied by a multi-dimensional stress response were found. Analysis of transcription factors showed that most of the down-regulated transcription factors were related to growth and development, and the up-regulated with abiotic and biotic stress responses and changes in developmental programs. Interestingly, the expression of most cell cycle related genes did not change significantly. Transcriptome analysis provided a successful approach to identify genes involved in mannitol treatment, essential for triggering microspore embryogenesis. María Muñoz-Amatriaín is recipient of a FPI fellowship and a Short-term Scientific Mission to UCR, from Ministerio de Educación y Ciencia of Spain. The research was supported by Projects AGL-2001-1631 and AGL-2004-03396, from Plan Nacional I+D+I, Ministerio de Educación y Ciencia of Spain. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3643 |
| Other Identifiers: | Physiologia Plantarum 127 (4) , 551–560 0031-9317 http://hdl.handle.net/10261/3643 10.1111/j.1399-3054.2006.00729.x |
| Appears in Collections: | Digital Csic |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.