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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Yruela Guerrero, Inmaculada | - |
| dc.creator | García-Rubio, Inés | - |
| dc.creator | Roncel Gil, Mercedes | - |
| dc.creator | Martínez, Jesús I. | - |
| dc.creator | Ramiro Pascual, María Victoria | - |
| dc.creator | Ortega, José M. | - |
| dc.creator | Alonso, Pablo J. | - |
| dc.creator | Picorel Castaño, Rafael | - |
| dc.date | 2008-04-16T15:43:20Z | - |
| dc.date | 2008-04-16T15:43:20Z | - |
| dc.date | 2003 | - |
| dc.date.accessioned | 2017-01-31T01:02:28Z | - |
| dc.date.available | 2017-01-31T01:02:28Z | - |
| dc.identifier | Photochem. Photobiol. Sci., 2003, (4),437-442 | - |
| dc.identifier | http://hdl.handle.net/10261/3630 | - |
| dc.identifier | 10.1039/b300187c | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3630 | - |
| dc.description | The definitive version is available at: http://www.rsc.org/publishing/journals/PP/ | - |
| dc.description | The detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1–D2–Cyt b559 complex preparations. Various n-dodecyl--D-maltoside concentrations from 0 to 0.2%(w/v) were used to stabilise the D1–D2–Cyt b559 complexes. Low spin heme EPR spectra were obtained but the gz feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (Em=+123–150 mV) in all the D1–D2–Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein. | - |
| dc.description | I. G.-R. was recipient of a fellowship from the Ministry of Education and Culture of Spain. This work was supported by the Dirección General de Investigación Científica y Técnica (Grants PB98-1632 to R.P. and PB97-1135 to J.M.O.) and by the Diputación General de Aragón (Projects P17/98 to P.J.A. and P111/2001 to J.I.M.). | - |
| dc.description | Peer reviewed | - |
| dc.format | 158025 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Royal Society of Chemistry (Great Britain) | - |
| dc.rights | openAccess | - |
| dc.subject | cytochrome b559 | - |
| dc.subject | detergent | - |
| dc.subject | electron paramagnetic resonance | - |
| dc.subject | photosystem II | - |
| dc.subject | reaction centre | - |
| dc.subject | redox potential | - |
| dc.title | Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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