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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3589| Title: | Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system |
| Keywords: | Cerastes vipera Macrovipera lebetina transmediterranea Integrin inhibition Snake venom disintegrin Snake venomics Toxin evolution |
| Publisher: | Biochemical Society |
| Description: | Copyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htm We report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs of short disintegrins and those coding for precursors of dimeric disintegrin chains. The Macrovipera lebetina transmediterranea dimeric disintegrin reported to inhibit the laminin-binding integrins α3β1, α6β1 and α7β1 was analysed using a proteomic approach and was shown to bear MLD (Met-Leu-Asp) and VGD (Val-Gly-Asp) motifs. The results highlight the fact that disintegrins have evolved a restricted panel of integrin-blocking sequences that segregate with defined branches of the phylogenetic tree of the integrin α-chains, providing novel insights into the evolutionary adaptation of the snake venom antagonists to the ligand-binding sites of their target integrin receptors. This study has been partly financed by grant BFU2004-01432/BMC from the Ministerio de Educación y Ciencia, Madrid, Spain, and by the Spanish–Tunisian Cooperation Programme 29p/02 from the Agencia Española de Cooperación Internacional (AECI). Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3589 |
| Other Identifiers: | Biochem J. 2006 April 15; 395(Pt 2): 385–392 1470-8728 http://hdl.handle.net/10261/3589 10.1042/BJ20051678 |
| Appears in Collections: | Digital Csic |
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