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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3266| Title: | Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris |
| Keywords: | Tick infestations Rhipicephalus (Boophilus) spp. tick Vaccines Recombinant DNA technology Pichia pastoris Animal health |
| Publisher: | BioMed Central |
| Description: | [Background] Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The R. microplus Bm86 protective antigen has been produced by recombinant DNA technology and shown to protect cattle against tick infestations. [Results] In this study, the genes for Bm86 (R. microplus), Ba86 (R. annulatus) and Bd86 (R. decoloratus) were cloned and characterized from African or Asian tick strains and the recombinant proteins were secreted and purified from P. pastoris. The secretion of recombinant Bm86 ortholog proteins in P. pastoris allowed for a simple purification process rendering a final product with high recovery (35–42%) and purity (80–85%) and likely to result in a more reproducible conformation closely resembling the native protein. Rabbit immunization experiments with recombinant proteins showed immune cross-reactivity between Bm86 ortholog proteins. [Conclusion] These experiments support the development and testing of vaccines containing recombinant Bm86, Ba86 and Bd86 secreted in P. pastoris for the control of tick infestations in Africa. This work was supported by the Wellcome Trust under the Animal Health in the Developing World initiative through project 0757990 entitled "Adapting recombinant anti-tick vaccines to livestock in Africa" and the Consejería de Educación y Ciencia, JCCM, Spain (project PAI06-0046-5285) and was facilitated through the Integrated Consortium on Ticks and Tick-borne Diseases (ICTTD-3), financed by the International Cooperation Program of the European Union, coordination action project No. 510561. V. Naranjo was funded by Junta de Comunidades de Castilla–La Mancha (JCCM), Spain. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3266 |
| Other Identifiers: | BMC Biotechnology 2008, 8:14 1472-6750 http://hdl.handle.net/10261/3266 10.1186/1472-6750-8-14 |
| Appears in Collections: | Digital Csic |
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