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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3051Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Nissen, Lorenzo | - |
| dc.creator | Yebra, María Jesús | - |
| dc.creator | Sgobarti, Barbara | - |
| dc.creator | Pérez Martínez, Gaspar | - |
| dc.date | 2008-02-25T10:36:31Z | - |
| dc.date | 2008-02-25T10:36:31Z | - |
| dc.date | 2005-04 | - |
| dc.date.accessioned | 2017-01-31T01:00:21Z | - |
| dc.date.available | 2017-01-31T01:00:21Z | - |
| dc.identifier | Biofutura 2005, Bologna, 8-10 Aprile 2005 | - |
| dc.identifier | http://hdl.handle.net/10261/3051 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3051 | - |
| dc.description | Sorbitol is a sugar polyol claimed to have health-promoting properties. D-sorbitol-6-phosphate dehydrogeanse (StolPDh) is required for sorbose and sorbitol metabolism in Lactobacillus casei. StolPDh catalyzes the oxidation of Sorbitol-6-phosphate and also the reverse reaction, or reather, the reduction of fructose-6-phosphate with NAD+ regeneration. In order to test this function in vivo, a new food grade recombinant strain of L. casei was constructed by the integration of a StolPDh-encoding gene (gutF) in the chromosomal lactose operon. Lactose induction of gutF expression was achieved and sorbitol production could be detected by 13C-nuclear magnetic resonance analys, upon addiction of glucose to resting cells. Additional inactivationn of L-lactate dehydrogenase led to higher sorbitol production. These outcomes indicates that the accumulation of the glycolytic precursor, fructose-6-phosphate and an high NAD+/NADH ratio were the limiting factors for sorbitol accumulation. | - |
| dc.format | 213952 bytes | - |
| dc.format | 2459 bytes | - |
| dc.format | application/pdf | - |
| dc.format | text/plain | - |
| dc.language | eng | - |
| dc.publisher | Associazione Nazionale Biotecnologi Italiani | - |
| dc.rights | openAccess | - |
| dc.subject | Lactobacillus casei | - |
| dc.subject | Sorbitol | - |
| dc.title | Engineering sorbitol-6-phosphate Dehydrogenase encoding gene in the lactose operon of Lactobacillus casei. | - |
| dc.type | Presentación | - |
| Appears in Collections: | Digital Csic | |
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