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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2972| Title: | Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide |
| Keywords: | Fructose-1,6-biphosphate Glyceraldehyde- 3-phosphate dehydrogenase Intestinal preconditioning Ischaemia/reperfusion injury Nitric Oxide |
| Publisher: | BMJ Publishing Group |
| Description: | [BACKGROUND AND AIMS] Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by nitric oxide (NO) in intestinal preconditioning could modify the rate of formation of glycolytic intermediates. Fructose-1,6-biphosphate (F16BP) is a glycolytic intermediate that protects tissue from ischaemia/reperfusion injury. We evaluated if F16BP may be endogenously accumulated as a consequence of GAPDH inhibition by NO during intestinal preconditioning in rats. [METHODS] We assessed: (1) effect of preconditioning on F16BP content; (2) effect of NO on GAPDH activity before and during sustained ischaemia; and (3) protective effect of F16BP in control, ischaemic, and preconditioned animals with or without administration of N-nitro-L-arginine methyl ester (L-NAME), NO donor, or F16BP. [RESULTS] Preconditioned rats showed a significant transient decrease in GAPDH activity and also maintained basal F16BP levels longer than ischaemic rats. L-NAME administration to preconditioned rats reversed these effects. F16BP administration to ischaemic rats decreased protein release in the perfusate. Administration of F16BP to L-NAME treated rats attenuated the harmful effect of L-NAME. [CONCLUSIONS] Our study indicates that F16BP may be endogenously accumulated in preconditioned rats as a consequence of inhibition of GAPDH by NO, and this may contribute to the protection observed in intestinal preconditioning. This work was supported by grant FIS 98/002901. A Sola was supported by a grant from Institut d’Investigacions Biomèdiques August Pi I Sunyer. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2972 |
| Other Identifiers: | Gut. 2001 February; 48(2): 168–175. http://dx.doi.org/10.1136/gut.48.2.168 0017-5749 http://hdl.handle.net/10261/2972 10.1136/gut.48.2.168 |
| Appears in Collections: | Digital Csic |
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