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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2971Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Serrano-Mollar, Anna | - |
| dc.creator | Closa, Daniel | - |
| dc.creator | Morcillo, Esteban J. | - |
| dc.creator | Bulbena, Oriol | - |
| dc.date | 2008-02-18T18:03:08Z | - |
| dc.date | 2008-02-18T18:03:08Z | - |
| dc.date | 2003-03-26 | - |
| dc.date.accessioned | 2017-01-31T01:00:10Z | - |
| dc.date.available | 2017-01-31T01:00:10Z | - |
| dc.identifier | British Journal of Pharmacology 138(6): 1037–1048 (2003) | - |
| dc.identifier | 0007-1188 | - |
| dc.identifier | http://hdl.handle.net/10261/2971 | - |
| dc.identifier | 10.1038/sj.bjp.0705138 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2971 | - |
| dc.description | This study examines the activity of the antioxidant N-acetylcysteine on bleomycin-induced pulmonary fibrosis in rats with emphasis on the early inflammatory phase. Rats receiving N-acetylcysteine (300 mg kg−1 day−1, intraperitoneal) had less augmented lung wet weight, and lower levels of proteins, lactate dehydrogenase, neutrophil and macrophage counts in bronchoalveolar lavage fluid and lung myeloperoxidase activity with a betterment of histological score at 3 days postbleomycin. A diminished lung GSH/GSSG ratio and augmented lipid hydroperoxides were observed 3 days postbleomycin. These changes were attenuated by N-acetylcysteine. Alveolar macrophages from bleomycin-exposed rats released augmented amounts of superoxide anion and nitric oxide. N-Acetylcysteine did not modify superoxide anion generation but reduced the increased production of nitric oxide. N-Acetylcysteine suppressed the bleomycin-induced increased activation of lung NF-κB (shift assay and immunohistochemistry), and decreased the augmented levels of the early inflammatory cytokines, tumour necrosis factor-α, interleukin-β, interleukin-6 and macrophage inflammatory protein-2 observed in bronchoalveolar lavage fluid at 1 and 3 days postbleomycin exposure. At 15 days postbleomycin, N-acetylcysteine decreased collagen deposition in bleomycin-exposed rats (hydroxyproline content: 6351±669 and 4626±288 μg per lung in drug vehicle- and N-acetylcysteine-treated rats, respectively; P<0.05). Semiquantitative histological assessment at this stage showed less collagen deposition in N-acetylcysteine-treated rats compared to those receiving bleomycin alone. These results indicate that N-acetylcysteine reduces the primary inflammatory events, thus preventing cellular damage and the subsequent development of pulmonary fibrosis in the bleomycin rat model. | - |
| dc.description | This work was supported by Grant 1FD97-1143 from the European Union (Regional Development Funds, FEDER), CICYT (Spanish Government), Regional Government (Generalitat Valenciana) and Grant FIS98/1367 (Spanish Ministry of Health). | - |
| dc.description | Peer reviewed | - |
| dc.format | 481474 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Wiley-Blackwell | - |
| dc.relation | http://dx.doi.org/10.1038/sj.bjp.0705138 | - |
| dc.rights | openAccess | - |
| dc.subject | Pulmonary fibrosis | - |
| dc.subject | Bleomycin | - |
| dc.subject | Rat | - |
| dc.subject | N-acetylcysteine | - |
| dc.subject | Inflammation | - |
| dc.title | In vivo antioxidant treatment protects against bleomycin-induced lung damage in rats | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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