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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2421| Title: | Utilisation of rapeseed protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity Utilización de aislados proteicos de colza para la producción de péptidos con actividad inhibidora de la enzima convertidora de la angiotensina (ECA) |
| Keywords: | Angiotensin converting enzyme Peptide inhibitors Protein hydrolysate Protein isolate Rapeseed |
| Publisher: | Consejo Superior de Investigaciones Científicas (España) |
| Description: | ACE activity is related to increased arterial pressure and coronary diseases. A rapeseed protein isolate was hydrolyzed
with the protease Alcalase in order to investigate the possible presence of ACE inhibitory peptides in the resulting hydrolysates. Hydrolysis for 30 min yielded a hydrolysate with the highest ACE
inhibitory activity. Two fractions of this hydrolysate obtained by Biogel P2 gel filtration chromatography were used for further purification of ACE inhibitory peptides. Three fractions with ACE
inhibitory activity were purified by reverse-phase HPLC of Biogel P2 f ractions. This demonstrates that rapeseed protein
hydrolysates represent a good source of ACE inhibitory peptides. La actividad de ECA está relacionada con una presión arterial alta y enfermedades cardíacas. Un aislado proteico de colza se hidrolizó con alcalasa para estudiar la posible presencia de péptidos inhibidores de ECA en el hidrolizado. La hidrólisis durante 30 min produjo el hidrolizado con la mayor actividad inhibidora de ECA. Dos fracciones de este hidrolizado, obtenidas por cromatografía de filtración en gel Biogel P2, se usaron para la purificación de péptidos inhibidores de ECA. Tres fracciones con actividad inhibidora de ECA se purificaron mediante HPLC en fase reversa de las fracciones obtenidas mediante Biogel P2. Esto demuestra que los hidrolizados proteicos de colza representan una buena fuente de péptidos inhibidores de ECA. This work was supported by research grant AGL 2001-0526 (F.M.), AGL 2002-02836 (J.G.C.) and a Ramón y Cajal contract (J.G.C.). Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2421 |
| Other Identifiers: | Grasas y aceites 55 (4): 354-358 (2004) 1988-4214 http://hdl.handle.net/10261/2421 |
| Appears in Collections: | Digital Csic |
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