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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2194| Title: | Ageing induces apoptosis and increases HSP70 stress protein in the epididymis of Octodon degus El envejecimiento induce apoptosis y aumento de la proteína HSP70 en el epidídimo de Octodon degus |
| Keywords: | Androgens Apoptosis Epididymis ageing Heat Shock Proteins |
| Publisher: | Sociedad Chilena de Anatomía |
| Description: | Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other hand, we used a immunocytochemical method to localize the stress protein HSP70. HSP70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. Changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenon. La apoptosis ha sido muy estudiada en el testículo, no así en el epidídimo. Se analiza el número de células apoptóticas en las diferentes regiones epididimarias (cabeza, cuerpo, cola) del roedor sudamericano Octodon degus joven y senil. La apoptosis se identificó con la técnica de TUNEL que detecta fragmentación del ADN y se la observó en las células epiteliales principales. La cabeza fue el segmento más afectado, con 0.32/1000 células apoptóticas en los animales jóvenes vs 5.1/1000 en seniles. También en la cola la apoptosis aumenta con la edad, con 0.14/1000 y 3.9/1000 en jóvenes y seniles, respectivamente. Por otra parte, la detección inmunohistoquímica de la proteína de estrés termico HSP-70 mostró que ésta aumenta notoriamente en las células principales de la cola epididimaria en animales seniles. Los cambios en el epitelio epididimario se deben probablemente a los bajos niveles de andrógenos del animal senil y son dependientes de la región epididimaria analizada. This work was supported by a Convenio de Colaboración CSIC-Universidad de Chile, and by Grant BCM2000-0899 from the DGICYT (Spain). Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2194 |
| Other Identifiers: | International Journal of Morphology, 2004, vol.22, no.1, p.29-34. 0717-9502 http://hdl.handle.net/10261/2194 |
| Appears in Collections: | Digital Csic |
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