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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Montañez Sáenz, Julio | - |
| dc.creator | Téllez, Alejandro | - |
| dc.creator | Garza, Heliodoro de la | - |
| dc.creator | Reyes, María de la Luz | - |
| dc.creator | Contreras-Esquivel, Juan Carlos | - |
| dc.creator | Aguilar, Cristóbal Noé | - |
| dc.date | 2007-11-15T15:50:16Z | - |
| dc.date | 2007-11-15T15:50:16Z | - |
| dc.date | 2000 | - |
| dc.date.accessioned | 2017-01-31T00:58:41Z | - |
| dc.date.available | 2017-01-31T00:58:41Z | - |
| dc.identifier | Braz. arch. biol. technol., 2000, vol.43, no.4, p.0-0 | - |
| dc.identifier | 1516-8913 | - |
| dc.identifier | http://hdl.handle.net/10261/2173 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2173 | - |
| dc.description | Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel | - |
| dc.description | Authors wish to thank National Council for Science & Technology (CONACYT, México 83076/124550). This research was partially supported by the CGEPI-UAdeC and forms part of the thesis work of Julio Cesar Montañez. Authors wish to thank researchers of PP-4 of Biotechnology Dept, in the Universidad Autonoma Metropolitana Iztapalapa for facilities and critical opinions offered. | - |
| dc.description | Peer reviewed | - |
| dc.language | eng | - |
| dc.publisher | Instituto de Tecnologia do Paraná | - |
| dc.rights | openAccess | - |
| dc.subject | Extraction | - |
| dc.subject | Purification | - |
| dc.subject | Properties | - |
| dc.subject | Pectinesterase | - |
| dc.subject | Potato | - |
| dc.title | Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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